Certificate

Lot # NH175508

Catalog #

Product FAQ

What concentration of my antibody should I use for cell analysis?

Answer

An optimal concentration may be between 1-10 μg/mL for cell and tissue labeling for microscopy, or 0.2-5 μg/mL for flow cytometry. A range of concentrations should be tested to determine what is optimal.

Answer Id: E14774

Was this answer helpful?

Yes
No
Thank you for your response

Certificate

Lot # 1021807-COC

Catalog #

Product FAQ

I have a very low-abundance antigen. How can I amplify my signal?

Answer

A common method for amplifying antibody detection is biotin-streptavidin detection, where a biotinylated secondary antibody is combined with subsequent labeling with a dye-conjugated streptavidin. This will amplify the signal by approximately 2-8 times, but endogenous biotin must be blocked beforehand. Another option is to use tyramide-signal amplification, where a horseradish peroxidase conjugate is used with a dye-labeled tyramide. This will amplify the signal by approximately 10-20 times, but endogenous peroxidase will need to be blocked. A final option may be to use a Qdot nanoparticle antibody or streptavidin conjugate, which can yield a signal as much as 40 times higher than a standard organic dye conjugate, depending on the Qdot color.

Answer Id: E14775

Was this answer helpful?

Yes
No
Thank you for your response

Certificate

Lot # 1149069-COA

Catalog #

Certificate

Lot # 1141671

Catalog #

Certificate

Lot # 1128579-COC

Catalog #

Product FAQ

Where can I go to get help with choosing dyes for labeling organelles and cell structures?

Answer

Visiting the imaging cell structure page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure.html) will help you get started choosing the best dyes and reagents for organelle labeling. Our Cell Staining Simulation tool (https://www.thermofisher.com/us/en/home/life-science/lab-data-management-analysis-software/lab-apps/cell-staining-tool.html) is also helpful, where you can see an example simulated cell image as you pick and choose reagents.

Answer Id: E14776

Was this answer helpful?

Yes
No
Thank you for your response

Certificate

Lot # 623038-COA

Catalog #
  • BC0100M-TS

Certificate

Lot # 1215940-COA

Catalog #
  • BO1226B-TS

Certificate

Lot # 757666-COA

Catalog #

Certificate

Lot # OK193469

Catalog #

Certificate

Lot # RL2305704

Catalog #

Product FAQ

DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?

Answer

DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.

Answer Id: E14777

Was this answer helpful?

Yes
No
Thank you for your response

Certificate

Lot # TC2541486

Catalog #