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super bright dyes

Product FAQ

Are the Super Bright dyes sensitive to methanol fixation?

Answer

Super Bright dyes are stable in methanol-based fixation buffers.

Answer Id: E14686

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Are the Super Bright Dyes photo-labile?

Answer

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.

Answer Id: E14441

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Product FAQ

What are the Super Bright dyes?

Answer

eBioscience Super Bright dyes are a series of patent-pending fluorochromes that are based upon a fluorescent polymer and its tandems. Super Bright dyes have been developed for use in flow cytometry and can be excited by the violet laser (405 nm). The peak emission of each Super Bright dye is indicated by the number in the product description, e.g., "Super Bright 600" has a peak emission of 600 nm.

Answer Id: E14676

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Can the Super Bright dyes be used for non-flow applications?

Answer

Super Bright dyes have not been tested for applications other than flow cytometry.

Answer Id: E14678

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Product FAQ

Can I use the OneComp and UltraComp eBeads™ microspheres with Super Bright-conjugated antibodies?

Answer

UltraComp eBeads™ microspheres (Cat. No. 01-2222) are recommended for use with Super Bright dyes.
Note: Super Bright Staining Buffer (Cat. No. SB-4400) is not compatible with UltraComp eBeads microspheres (Cat. No. 01-2222-41, 00-2222-42). If using UltraComp eBeads microspheres as a compensation tool, solely use Flow Cytometry Stain Buffer (Cat. No. 00-4222-26, 00-4222-57) for any antibody dilutions.

Answer Id: E14689

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Product FAQ

How does the protocol for staining with Super Bright-conjugated antibodies differ from staining with conventional organic fluorochrome and eVolve™ conjugated antibodies?

Answer

Antibodies conjugated to Super Bright dyes can be used similarly to traditional fluorochromes. If multiple Super Bright dyes are used in combination with each other or in combination with other polymer dyes (i.e., Brilliant Violet™ dyes), then the use of Super Bright Staining Buffer (Cat. No. SB-4400) is recommended to minimize any non-specific polymer interactions between these fluorochromes. For specific instructions for use, please refer to the product technical data sheet.

Answer Id: E14679

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Product FAQ

Can I fix my cells after staining with Super Bright-conjugated antibodies? How long can fixed cells be stored prior to analysis?

Answer

Samples that have been stained with antibodies conjugated to Super Bright dyes may be stored for up to three days, at 2-8°C, in the dark, using either IC Fixation Buffer (Cat. No. 00-8222) or 1-step Fix/Lyse Buffer (Cat. No. 00-5333) with no significant effect on brightness or compensation.

Answer Id: E14687

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Product FAQ

Can Super Bright-conjugated antibodies be used in combination with other polymer dyes? What buffer should I use when using more than one Super Bright or polymer dye?

Answer

When using two or more Super Bright dyes, or when using Super Bright dyes in combination with other polymer dyes (i.e., Brilliant Violet™ dyes), we recommend using the Super Bright Staining Buffer (Cat. No. SB-4400) to minimize any non-specific polymer interactions between these fluorochromes. For specific instructions for use, please refer to the product technical data sheet.

Answer Id: E14682

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Product FAQ

What is the difference between the Super Bright and eVolve™ dyes?

Answer

Super Bright dyes are fluorochromes based on polymer technology and are excited by the violet laser. In contrast, eVolve dyes are based on Qdot™ nanocrystal technology and are maximally excited by the UV laser, although they may also be excited by violet, blue, yellow-green, and, depending on the eVolve, the red laser lines. The general shape of the emission spectra for Super Bright dyes will be more similar to traditional fluorochromes, while eVolve dyes have extremely narrow emission spectra. Please contact Tech Support (techsupport@thermofisher.com) for more information.

Answer Id: E14677

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Product FAQ

Is there a specific buffer I should use when staining with Super Bright-conjugated antibodies?

Answer

No special buffer is required when using a single Super Bright-conjugated antibody in a panel.
When using more than one Super Bright dye, or when using Super Bright dyes in combination with other polymer dyes (i.e., Brilliant Violet™ dyes), we recommend using the Super Bright Staining Buffer (Cat. No. SB-4400) to minimize any non-specific polymer interactions between these fluorochromes. For specific instructions for use, please refer to the product technical data sheet.

Answer Id: E14680

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Product FAQ

Can the Super Bright Staining Buffer be used with other polymer dyes?

Answer

Yes, the Super Bright Staining Buffer (Cat. No. SB-4400) is compatible with other polymer dyes (i.e., Brilliant Violet™ dyes) and is useful for minimizing any non-specific polymer interactions when two or more of these dyes are used in combination.

Answer Id: E14683

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Product FAQ

Which fluorochromes are recommended for antibody staining in the PrimeFlow™ RNA Assay?

Answer

Most organic and protein-based fluorochromes are compatible with this assay kit, including PE, PE tandems, APC, APC tandems, and small organic dyes such as FITC, eFluor™ 450, eFluor™ 506, eFluor™ 660, and Alexa Fluor™ 700. However, PerCP, PerCP-Cyanine5.5, and PerCP-eFluor™ 710 may not be used, and we recommend using PE-Cyanine5 or PE-Cyanine5.5 instead. Qdot™ nanocrystal and eVolve™-antibody conjugates are not compatible with this assay. If Super Bright or Brilliant Violet conjugated antibodies will be utilized, please contact Tech Support at techsupport@thermofisher.com for assistance with optimizing your multicolor panel.

Answer Id: E14578

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