Product FAQ

What is the difference between Invivofectamine™ 2.0 Reagent and Invivofectamine™ 3.0 Reagent?

Answer

Invivofectamine™ 3.0 Reagent is an improved formulation with the following increased benefits:

- Lower toxicity
- Higher transfection efficiency with less siRNA; sustained knockdown up to 2 weeks
- Increased stability
- Shorter overall workflow; no dialysis or diafiltration needed

Answer Id: E10382

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Product FAQ

What is the Invitrogen™ Lipofectamine™ MessengerMAX™ Transfection Reagent?

Answer

Lipofectamine™ MessengerMAX™ Transfection Reagent is an animal origin-free transfection reagent, especially formulated for the delivery of mRNA, small RNA (eg.,CRISPR IVT gRNA, siRNA, or miRNA), and short dsDNA or HDR templates (0.5-1 kb). Lipofectamine™ MessengerMAX™ Reagent is an excellent reagent choice for CRISPR-mediated genome editing applications.

Answer Id: E10383

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Product FAQ

When would I use Lipofectamine™ MessengerMAX™ Reagent over other transfection reagents?

Answer

Lipofectamine™ MessengerMAX™ Regent is an excellent reagent for co-delivery of Invitrogen™ GeneArt™ CRISPR Cas9 mRNA with in vitro transcribed gRNA for geneome editing purposes. Lipofectamine™ MessengerMAX™ Reagent has the added benefit of flexibly delivering short dsDNA or HDR templates (0.5-1 kb) which can be ordered through the Invitrogen™ GeneArt™ Strings services.

Answer Id: E10384

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Product FAQ

What storage buffer do you recommend for the SUPERase.In™ RNase Inhibitor?

Answer

The storage buffer we recommend is as follows: 2 mM KH2PO4, 8 mM Na2HPO4, 2.7 mM KCl, 137 mM NaCl, pH 7.4 in 50% glycerol.

Answer Id: E13227

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Product FAQ

Which cell types can be used with Lipofectamine™ MessengerMAX™ Transfection Reagent?

Answer

Lipofectamine™ MessengerMAX™ Reagent demonstrates low toxicity and high transfection efficiency for all cell types (easy or difficult-to-transfect, primary, and stem cells).

Answer Id: E10385

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Product FAQ

Is it normal to see line to line variability in cardiac differentiation?

Answer

Yes. Variability is normal and it is not uncommon to find certain lines that will not differentiate as efficiently. Including a control line, such as the human ESC H1 or H9 cells, which have been shown to differentiate consistently well, may be helpful.

Answer Id: E10403

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Product FAQ

What concentration should the UltraPure™ Salmon Sperm DNA Solution (Cat. No. 15632011) be used at?

Answer

Carrier DNA is typically used at a concentration of 100 μg/mL in both the prehybridization and hybridization solutions.

Answer Id: E13228

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Product FAQ

How do I use Lipofectamine™ MessengerMAX™ Reagent to deliver both my GeneArt™ Cas9 mRNA and IVT gRNA together?

Answer

Lipofectamine™ MessengerMAX™ Reagent may be used for both single gRNA delivery or multiplexing (gRNA for multiple targets) purposes with high transfection efficiency when used with GeneArt™ Cas9 mRNA. For a detailed protocol, please refer to the GeneArt™ Cas9 mRNA manual (https://www.thermofisher.com/order/catalog/product/A25640?ICID=search-a25640). We recommend using either the Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit (Cat. No. A24732) or Invitrogen™ GeneArt™ Genomic Cleavage Selection Kit (Cat. No. A27663) for mutant analysis.

Answer Id: E10386

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Product FAQ

What size of cells can the Invitrogen™ Countess™ II Automated Cell Counter and Invitrogen™ Countess™ II FL Automated Cell Counter count?

Answer

The Countess™ II and Countess™ II FL instruments can detect particles/cells ranging from 4 - 60 µm. Cells ranging from 7 - 60 µm can be counted and assessed for viability with brightfield viewing.

Answer Id: E10404

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Product FAQ

Which RNases does Thermo Scientific RiboLock RNase Inhibitor inhibit?

Answer

Thermo Scientific RiboLock RNase Inhibitor inhibits the activity of RNases A, B, and C. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 or prokaryotic RNases I and H.

Answer Id: E13229

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Product FAQ

Can I deliver single-stranded oligonucleotides with Lipofectamine™ MessengerMAX™ Transfection Reagent?

Answer

We recommend using Invitrogen™ Lipofectamine™ RNAiMAX Reagent for the delivery of oligos. We do not recommend using Lipofectamine™ MessengerMAX™ Reagent for the delivery of single-stranded oligonucleotides for genome editing purposes; literature demonstrates that dsDNA HDR templates provide better directed results.

Answer Id: E10387

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Product FAQ

How long do the Invitrogen™ Countess™ II and Invitrogen™ Countess™ II FL instruments take to count cells?

Answer

The time taken is ˜10 seconds per sample.

Answer Id: E10405

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Product FAQ

Will PCR amplifications previously successful with regular AmpliTaq™ Polymerase work as successfully with AmpliTaq Gold™ Polymerase?

Answer

They will work more successfully and reproducibly with AmpliTaq Gold™ Polymerase. Although AmpliTaq Gold™ Polymerase is the same exact enzyme as AmpliTaq™ Polymerase, the fact that the reaction is being driven towards high specificity and yield may require some modifications to previous conditions. For example, if the previous reaction was on the edge of optimization, magnesium chloride concentrations may need to be re-optimized or if previous reactions were being run in a pH suboptimal for AmpliTaq Gold™ Polymerase, reaction conditions and sample preparation protocols may need to be revisited. Activation time for AmpliTaq Gold™ Polymerase will also need to be determined empirically and is dependent on cycler type.

Answer Id: E1074

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Product FAQ

Do you offer a kit for isolation of intact mitochondria from cultured cells?

Answer

Yes we offer the Mitochondria Isolation Kit for Cultured Cells, Cat. No. 89874.

Answer Id: E13246

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Product FAQ

What is the best method to reamplify a gel-purified or non gel-purified PCR product, and not see a smear?

Answer

DNA concentration is a critical parameter in PCR amplification. If too much DNA is used, a smear will often result (due to concatamer formation of the target DNA that can occur at higher concentrations). When reamplifying PCR product, a good starting concentration is 10 pg. If the DNA cannot be quantified, it may be necessary to set up reamplification reactions using serial dilutions of the original product to obtain good amplification of a single-sized product.

Answer Id: E1322

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