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Maintenance and Troubleshooting Guide: 3730/3730xl DNA Analyzer

Manual / Product Insert

Version: 4359473 Rev. B (April 2005)

PI: POP-7th Polymer - CG - For the 3730 Series Genetic Analyzer

Manual / Product Insert

Version: 4487170 Rev. A 18July2013
Catalog #

How can a solid yellow light on the Applied Biosystems™ 3730 Series DNA Analyzer be resolved?

Product FAQ

Answer

If a solid yellow light remains on the Applied Biosystems™ 3730 Series DNA Analyzer, perform the following steps:

1) Cycle power on the system in the proper order:
     a. Boot/Reboot the computer workstation and log in to the computer workstation
     b. Ensure that the buffer, water, and waste reservoirs are in their appropriate stations.
     c. Close the oven door, stacker drawer, and instrument doors.
     d. Cycle power on the Applied Biosystems™ 3730 Series DNA Analyzer.

2) Confirm that the original communication cable is attached properly ("computer" labeled end attached to computer and "instrument" labeled end attached to instrument). NOTE: the original communication cable must be used as it is of a non-standard design.

3) Reset the "3730User" account password. To do so:
     a. Right click on "My Computer" and click on "Manage".
     b. In the Computer Management window, click on "Local Users and Groups" and click on "User".
     c. On the right side of the window, there should be a username "3730User" Right click on that and select "Set Password".
     d. Set the password to "3730User" (case sensitive). Confirm the password entry then click on "Ok".
     e. In the Computer Management window, right click on "3730User" and select "Properties". "User cannot change password" and "Password never expires" should be selected.
     f. Click on "Ok" and exit out of Computer Management window.

4) Confirm whether or not your IT department has:
     a. Changed the name of the computer workstation.
     b. Installed or configured firewall/antiviral software on the computer workstation.
     c. Altered the original IP address of the computer/network card.
     d. Configured the computer for network access.
     e. Installed any updates or patches for the Operating System/Service Pack. NOTE: These changes may disrupt communication between the instrument and the computer.

5) Ensure that the "3730calib.ini" file is in the appropriate place. The file location on the Applied Biosystems™ 3730 Series DNA Analyzer running Data Collection v3.0 and v.3.1: C:/AppliedBiosystems/Firmware.

If the problems persist, contact Technical Support or Instrument Service.

Answer Id: E2546

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User Guide: Applied Biosystems 3730 3730xL DNA Analyzer

Manual / Product Insert

Version: 4331468 Rev.G (01Jan2015)

What are the sequencing performance specifications for the Applied Biosystems™ 3730 Series DNA Analyzer?

Product FAQ

Answer

On the 36 cm array:
- The Standard Sequencing run module has a run time of 60 minutes and an average read of 700 bases with a QV >20.
- The Rapid Sequencing run module has a run time of 35 minutes and an average read length of 550 bases with a QV >20.

On the 50 cm array:
- The Extra Long Read Sequencing run module has a run time of 180 minutes and an average read length of 900 bases with a QV >20.
- The Long Read Sequencing run module has a run time of 120 minutes and an average read length of 850 bases with a QV >20.
- The Fast Sequencing run module has a run time of 60 minutes and an average read length of 700 bases with a QV >20.

Answer Id: E2525

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