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Product FAQ

The amber light won't blink and remains at a solid amber light when I start the Applied Biosystems 3730/3730xl DNA Analyzer. What's wrong?

Answer

The amber light starts to blink once the instrument has logged onto the computer using the User account (e.g. 3730User). Anything that prevents it from connecting will cause the amber light to remain solid. Some of the more frequent reasons for this happening include:

-User password has expired or changed
-Firewall is on
-Cable is plugged into the wrong port
-The wrong network card was configured to connect to the local network
-Bad network card

Answer Id: E12743

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Product FAQ

Can I re-install the Applied Biosystems 3130/3130xl Genetic Analyzer Data Collection Software myself?

Answer

Installation of the Data Collection Software for the Applied Biosystems 3130/3130xl Genetic Analyzer is a complex process that requires a Field Service Engineer to install the software properly.

Answer Id: E12720

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Product FAQ

The amber light on my Applied Biosystems 3730/3730xl DNA Analyzer continues to blink and won't proceed to green like it normally does. What is wrong?

Answer

A perpetually blinking amber light usually indicates that there is a door open on the instrument or a bad door sensor. Open the main door, the oven door and the detector cell door (small black door with the laser warning on it) and close them again. After the autosampler stops moving the light should change to green. If the amber light does not go to green, it is possible that a door sensor is not working properly and a service call may be required.

Answer Id: E12744

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Product FAQ

What is the white residue around the Applied Biosystems3500/3500xL Genetic Analyzer array knob?

Answer

When placing the array on the instrument, the last step in the process is to loosen the array knob and the instrument forces any air bubbles around the array tip through the threading and the middle of the array knob. Some polymer gets pushed through as well and when it dries up, it appears as a white, flaky residue. Use a damp, lint-free cloth to remove it.

Answer Id: E12785

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Product FAQ

When should I perform a short-term shutdown of my Applied Biosystems 3130/3130xl Genetic Analyzer versus a long-term shutdown?

Answer

For the Applied Biosystems 3130/3130xl Genetic Analyzer, if the instrument will be idle for less than a week, perform the short-term shutdown of the system. If it will be idle for longer than a week, follow the instructions in the user guide for a long-term shutdown using the Instrument Shutdown Wizard.

Answer Id: E12689

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Product FAQ

How do I turn on my Applied Biosystems3500/3500xL Genetic Analyzer?

Answer

The Applied Biosystems3500 Genetic Analyzer system includes both the instrument and the computer - you cannot start or restart one without starting the other. Improper boot sequence can lead to communication issues, aborted runs, and possible loss of data. The proper boot sequence is as follows:

1. Turn on the computer and press on Ctrl + Alt + Del, then OK to get to the login screen but do not log in.
2. Press the power button to turn on the instrument and wait for the green light.
3. Log in to the computer with the INSTR-ADMIN user account and wait for the Integration Service to launch. When all of the services in the Integration Service are on, a green checkmark will appear over the icon.
4. Wait 1- 2 minutes to allow for programs to load in the background and then launch the Applied Biosystems3500 Series Data Collection Software.

Step-by-step instructions for the start-up procedures can be found in the Applied Biosystems3500/3500xL Genetic Analyzers: Starting Up the Instrument Technical Note (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/3500-Start-Up-Procedures.pdf) and the Applied Biosystems3500/3500xL Genetic Analyzer Getting Started Guide (Data Collection Software v.3.1)(http://www.thermofisher.com/us/en/home/technical-resources/technical-reference-library/capillary-electrophoresis-instruments-support-center/3500-3500xl-genetic-analyzers-support/3500-3500xl-genetic-analyzers-support-getting-started.html).

Answer Id: E12758

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Product FAQ

Can I purchase a computer from you or someone else and install the Applied Biosystems 3130/3130xl Genetic Analyzer software myself?

Answer

You can purchase a computer for installation of the secondary analysis software packages (e.g., Sequencing Analysis, GeneMapper software) as long as the computer meets the software computer requirements (for processor speed, the minimum speed of the processor should be at least 3 GHz). In these cases, you can install the software yourself.

For the instrument computer, the computer system has a much more stringent requirement and undergoes thorough testing to make sure it can handle the flow of data from the instrument to the computer. The images for the computer system are streamlined to make sure they have the proper drivers for the computer components, and the Operating System is optimized to make sure there is nothing running in the background that might slow the computer down, take up memory, or conflict with our software. Purchasing a computer commercially can introduce too many variables in terms of hardware and software that can negatively affect the data collection process so we cannot support connecting it to the instrument.

Answer Id: E12721

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Product FAQ

With my Applied Biosystems 3730/3730xl DNA Analyzer, I seem to be getting bubbles from the polymer bottle but the polymer level is normal. What is causing it?

Answer

It is possible that the polymer tube fitting may not be screwed into the pump properly, allowing air to get pulled in as the piston retracts. It is also possible that some debris may be stuck in the check valve, locking it in the open position. It may be necessary to detach and carefully attach the polymer tube again and then perform a water wash wizard to remove the bubbles and clear any debris from the check valve.

Answer Id: E12751

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Product FAQ

When I started my run on the Applied Biosystems 3730/3730xl DNA Analyzer, an error occurred: “Unstable Electrophoresis current detected, check for air bubbles” but I don't see any bubbles. What else can cause this?

Answer

Some of the other causes of an Unstable Electrophoresis current detected error message are:

1.Leak on the system
2.Polymer that:
-Has expired
-Has been left on the instrument for more than the recommended time
-Is a mixture of expired polymer and non-expired polymer
3.Using Running Buffer that was:
-Diluted to 1X incorrectly
-Swapped with the water position on the
Autosampler deck
-On the instrument longer than 48 hours
-Not filled to the fill line or evaporated below the fill line
4. An arcing event that was not cleaned afterwards using the water wash wizard
5. Not performing regular maintenance on the instrument
6. Hardware issues

Inspect the system for leaks. If you do not see any leaks on the system, perform a water wash using the water wash wizard and place fresh, non-expired polymer and freshly made 1X Running Buffer on the system. If the problem persists, a service call may be required.

Answer Id: E12745

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Product FAQ

Can I re-install the Applied Biosystems3500/3500xL Genetic Analyzer Data Collection Software myself?

Answer

Installation of the Data Collection Software for the Applied Biosystems3500/3500xL Genetic Analyzer Data Collection Software is a complex process that requires a Field Service Engineer to install the software properly

Answer Id: E12786

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Product FAQ

How do I shut off my Applied Biosystems 3130/3130xl Genetic Analyzer?

Answer

After performing the short-term or long-term shutdown wizards:

1. Click “Stop All” in the Service Console and wait until all 4 services turn green. Close the program.
2. Go to the Start menu and select Shut Down. On Windows 7, click the Windows icon in the lower left corner of the screen. Click the arrow next to “Sleep” and select “Shut Down”.
3. Click the power button on the instrument.

Step-by-step instructions for the shutdown procedures can be found in the Applied Biosystems 3130/3130xl Genetic Analyzers: Shutting Down the Instrument Technical Note (https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3130%20Shut%20Down%20Procedures.pdf) and the Applied Biosystems 3130/3130xl Genetic Analyzers Getting Started Guide (Data Collection Software v.4)(https://tools.thermofisher.com/content/sfs/manuals/4477796.PDF).

Answer Id: E12690

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Product FAQ

When should I perform a short-term shutdown of my Applied Biosystems3500/3500xL Genetic Analyzer versus a long-term shutdown?

Answer

For the Applied Biosystems3500 Series Genetic Analyzer, if the instrument will be idle for 1- 2 weeks, perform the short-term shutdown of the system. If the instrument will be idle for more than 2 weeks, perform the long-term shutdown using the Instrument Shutdown Wizard.

Answer Id: E12759

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Product FAQ

With my Applied Biosystems 3130/3130xl Genetic Analyzer, the start button is not active after linking my plate. Why?

Answer

A patch is available here(http://resource.thermofisher.com/pages/WE28396/) to fix this.

Answer Id: E12722

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Product FAQ

My array is stuck in the pump on my Applied Biosystems 3730/3730xl DNA Analyzer, even after loosening the knob. What can I do?

Answer

If the array is stuck in the pump, even after loosening the knob, squirt around the edge of the array head with distilled water and try to remove it again. If the array will not move, a service call may be required. Do not use excessive force or tools to try and remove it.

Answer Id: E12752

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Product FAQ

When I started my run on the Applied Biosystems 3730/3730xl DNA Analyzer, an error occurred: “Leak detected” but I don't see any leaks. What else might cause this?

Answer

“Leak detected” error messages can also occur when the piston on the pump moves further than expected during the movement of the pump. The usual cause for this is a leak on the system, usually due to either an improperly tightened or improperly threaded fitting or a broken capillary. However, if this is not observed, some of the other reasons are:

1.Valve stuck in open position
2.Large air bubble under the piston
3. Polymer:
-Wrong polymer type (e.g., instrument thinks it has POP-7 on it but it really has POP-4).
-Old/degraded polymer
4. Improper/no maintenance on the system

Inspect the system for leaks. If you do not see any leaks on the system, perform a water wash using the water wash wizard and place fresh, non-expired polymer and freshly made 1X Running Buffer on the system. If the problem persists, a service call may be required.

Answer Id: E12746

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