Catalog: Sample Handling Products Catalog 2016-2017
- Version: 17 May 2017
What is the composition of the NuPAGE™ LDS Sample Buffer and what is the recipe for the 4X formulation?
The composition of the 1X NuPAGE™ LDS Sample Buffer is as follows:
141 mM Tris base
106 mM Tris HCl
0.51 mM EDTA
0.22 mM SERVA Blue G
0.175 mM Phenol Red
To prepare 10 mL of 4 X NuPAGE™ LDS Sample Buffer, dissolve the following reagents to 8 mL ultrapure water:
Tris HCl 0.666 g
Tris Base 0.682 g
LDS 0.800 g
EDTA 0.006 g
Glycerol 4 g
SERVA Blue G (1 solution) 0.75 mL
Phenol Red (1 solution) 0.25 mL
Mix well and adjust the volume to 10 mL with ultrapure water. Store at +4. The buffer is stable for 6 months when stored at +4°C.
Answer Id: E4272
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Citations & References
Functional links between the fusion peptide-proximal polar segment and membrane-proximal region of human immunodeficiency virus gp41 in distinct phases of membrane fusion.
- Authors: Bellamy-McIntyre AK; Lay CS; Baar S; Maerz AL; Talbo GH; Drummer HE; Poumbourios P
- Journal: The Journal of Biological Chemistry 2007 32:23104-23116