Catalog: Sample Handling Products Catalog 2016-2017

Product Literature

Lot # HS250-666

Certificate

Lot # 666-101

Certificate

Catalog #

Lot # HS250-666

Certificate

Lot # HS250-666

Certificate

Lot # HS250-666

Certificate

Lot # HS250-666

Certificate

What is the composition of the NuPAGE™ LDS Sample Buffer and what is the recipe for the 4X formulation?

Product FAQ

Answer

The composition of the 1X NuPAGE™ LDS Sample Buffer is as follows:

141 mM Tris base
106 mM Tris HCl
2% LDS
10% Glycerol
0.51 mM EDTA
0.22 mM SERVA Blue G
0.175 mM Phenol Red
pH 8.5


To prepare 10 mL of 4 X NuPAGE™ LDS Sample Buffer, dissolve the following reagents to 8 mL ultrapure water:

Tris HCl 0.666 g
Tris Base 0.682 g
LDS 0.800 g
EDTA 0.006 g
Glycerol 4 g
SERVA Blue G (1 solution) 0.75 mL
Phenol Red (1 solution) 0.25 mL

Mix well and adjust the volume to 10 mL with ultrapure water. Store at +4. The buffer is stable for 6 months when stored at +4°C.

Answer Id: E4272

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Citations & References

  • Authors: Venier, P; De Pitta, C; Bernante, F; Varotto, L; De Nardi, B; Bovo, G; Roch, P; Novoa, B; Figueras, A; Pallavicini, A; Lanfranchi, G