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A27654SA

Product FAQ

How is PSC Definitive Endoderm Induction Kit shipped?

Answer

PSC Definitive Endoderm Induction Kit is shipped on dry ice. Upon receipt, store at -20 degrees C.

Answer Id: E9360

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Product FAQ

What is the recommended method to seed PSC to start Definitive Endoderm Induction?

Answer

When seeding PSCs for Definitive Endoderm induction, cells should be plated as very small clumps using Accutase Reagent. They can also be seeded as singularized cells using TrypLE Reagent. To promote cell survival, you can treat the cells overnight with ROCK inhibitors including RevitaCell Supplement , Y27632, or Thiazovivin.

Answer Id: E9384

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Product FAQ

How is the PSC Definitive Endoderm Induction Kit stored?

Answer

PSC Definitive Endoderm Induction Kit is supplied as two bottles of frozen media. Each should be stored at -20 degrees C, protected from light.

Answer Id: E9361

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Manual / Product Insert

Quick Ref: PSC Definitive Endoderm (DE) Induction Kit (Prototype)

Version: MAN0014009 Rev A.0 (5 May 2015)
Catalog #
  • A27654SA(Discontinued)

Product FAQ

I use TesR Medium as a PSC culture medium. Is PSC Definitive Endoderm Induction Medium compatible with this medium?

Answer

Optimal results are obtained by using Essential 8 Medium as a pluripotent stem cell medium, however mTesR1 or TeSR-E8 can be used as substitute, if desired.

Answer Id: E9385

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Product FAQ

Do I need to rinse cells between Definitive Endoderm Induction Medium A to Definitive Endoderm Induction Medium B switch?

Answer

No. It is not necessary to rinse cells when changing from Definitive Endoderm Induction Medium A to Definitive Endoderm Induction Medium B. However, Medium A should be completely removed before switching to Medium B.

Answer Id: E9388

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Product FAQ

What is the shelf life of this medium in the PSC Definitive Endoderm Induction Kit?

Answer

Shelf life for this product has not yet been established. However, in preliminary experiments, the product is shown to be stable for at least 6 months from time of manufacture when properly stored at -20 degrees C.

Answer Id: E9362

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Manual / Product Insert

User Guide: PSC Definitive Endoderm (DE) Induction Kit

Version: MAN0011159 Rev A.0 (05 February 2015)
Catalog #
  • A27654SA(Discontinued)

Product FAQ

What quality controls are used to ensure product performance for the PSC Definitive Endoderm Induction Kit?

Answer

Each lot is tested for % efficiency of definitive endoderm induction using flow cytometry analysis. Additionally, it is tested for sterility, endotoxin, pH, and osmolality.

Answer Id: E9370

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Product FAQ

Can the PSC Definitive Endoderm Induction Kit be used with species other than human?

Answer

Preliminary data have shown 82% Sox17 expression when the kit is used with mouse ESCs.

Answer Id: E9374

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Product FAQ

What dissociation reagent should I use with the PSC Definitive Endoderm Induction Kit?

Answer

For passaging PSCs we recommend EDTA, and for seeding cells for definitive endoderm induction we recommend Accutase Enzyme for small clumps and TrypLE Reagent for singularized cells.

Answer Id: E9382

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Product FAQ

At what seeding density should I seed PSCs prior to differentiation?

Answer

The optimum range for seeding density is 0.01 × 10e6-0.04 x 10e6 cells/cm2. For example seed 0.09 x 10e6 - 0.38 x 10e6 cells per well of a 6-well plate.

Answer Id: E9386

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Product FAQ

Can I purchase either Definitive Endoderm Induction Medium A or Definitive Endoderm Induction Medium B separately?

Answer

No. These media are not available separately.

Answer Id: E9368

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Product Literature

Pluripotent stem cell guidebook—key products and services for PSC research

Product FAQ

Are there any additional components that need to be added to the media of the PSC Definitive Endoderm Induction Kit?

Answer

No other components are required.

Answer Id: E9380

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