Thymosin ß10 expression driven by the human TERT promoter induces ovarian cancer-specific apoptosis through ROS production.

Citations & References

  • Authors: Kim YC, Kim BG, Lee JH,
  • Journal: PLoS One (2012) 7:e35399-e35399
  • PubMed ID: 22623951

I am using the reactive oxygen species detection dye, Dihydrorhodamine 123, and when I put the dye in a solution of water and illuminate with UV light, I see a quick change in signal, even without cells. What is happening and how can I stop this?

Product FAQ


You cannot stop it. Any time you illuminate a dye, including this one, photodegradation of the dye will lead to the production of singlet oxygen and free radicals. This in turn causes many ROS indicators, like this one, to become oxidized. If you are testing ROS using UV illumination, all you can do is use this as a baseline and subtract the signal from the sample with cells. The best you can do is reduce the illumination intensity (though this will also reduce the specific signal emission).

Answer Id: E6451

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CellROX Oxidative Stress Reagents

Manual / Product Insert

Version: 6 May 2012

Glutathione depletion is necessary for apoptosis in lymphoid cells independent of reactive oxygen species formation.

Citations & References

  • Authors: Franco R, Panayiotidis MI, Cidlowski JA,
  • Journal: J Biol Chem (2007) 282:30452-30465
  • PubMed ID: 17724027

Immunocytochemical localization of Pisum sativum TRXs f and m in non-photosynthetic tissues

Citations & References

  • Authors: Traverso, JA; Vignols, F; Cazalis, R; Serrato, AJ; Pulido, P; Sahrawy, M; Meyer, Y; Cejudo, FJ; Chueca, A
  • Journal: JOURNAL OF EXPERIMENTAL BOTANY 2008 6:1267-1277

Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response.

Citations & References

  • Authors: Frank G, Pressman E, Ophir R, Althan L, Shaked R, Freedman M, Shen S, Firon N
  • Journal: J Exp Bot 2009; (60):13 3891-3908
  • PubMed ID: 19628571

Cocaine-mediated enhancement of Tat toxicity in rat hippocampal cell cultures: the role of oxidative stress and D1 dopamine receptor.

Citations & References

  • Authors: Aksenov MY, Aksenova MV, Nath A, Ray PD, Mactutus CF, Booze RM
  • Journal: Neurotoxicology (2006) 27:217-228
  • PubMed ID: 16386305

Gene and protein expression in the epididymis of infertile c-ros receptor tyrosine kinase-deficient mice.

Citations & References

  • Authors: Cooper TG, Wagenfeld A, Cornwall GA, Hsia N, Chu ST, Orgebin-Crist MC, Drevet J, Vernet P, Avram C, Nieschlag E, Yeung CH
  • Journal: Biol Reprod 2003; (69):5 1750-1762
  • PubMed ID: 12890734

COX-2 induction by unconjugated bile acids involves reactive oxygen species-mediated signalling pathways in Barrett's oesophagus and oesophageal adenocarcinoma.

Citations & References

  • Authors: Song S, Guha S, Liu K, Buttar NS, Bresalier RS
  • Journal: Gut 2007 11:1512-1521
  • PubMed ID: 17604323

Cellular features of an apoptotic form of programmed cell death during the development of the ascidian, Boltenia villosa.

Citations & References

  • Authors: Bates WR
  • Journal: Zoolog Sci (2004) 21:553-563
  • PubMed ID: 15170059

Colony-Stimulating Factor-1 (CSF-1) Directly Inhibits Receptor Activator of Nuclear Factor-κB Ligand (RANKL) Expression by Osteoblasts

Citations & References

  • Authors: Wittrant, Y; Gorin, Y; Mohan, S; Wagner, B; Abboud-Werner, SL
  • Journal: Endocrinology 2009 11:4977-4988

Reoxygenation-specific activation of the antioxidant transcription factor Nrf2 mediates cytoprotective gene expression in ischemia-reperfusion injury.

Citations & References

  • Authors: Leonard MO; Kieran NE; Howell K; Burne MJ; Varadarajan R; Dhakshinamoorthy S; Porter AG; O'Farrelly C; Rabb H; Taylor CT
  • Journal: The Faseb Journal : Official Publication of the Federation of American Societies for Experimental Biology 2007 12:2624-2626

Thioredoxin-interacting protein mediates high glucose-induced reactive oxygen species (ROS) generation by mitochondria and the NADPH oxidase, Nox4, in mesangial cells.

Citations & References

  • Authors: Shah A, Xia L, Goldberg H, Lee KW, Quaggin SE, Fantus IG,
  • Journal: J Biol Chem (2013) 0:-
  • PubMed ID: 23329835

Rapid suppression of free radical formation by nerve growth factor involves the mitogen-activated protein kinase pathway.

Citations & References

  • Authors: Dugan LL, Creedon DJ, Johnson EM, Holtzman DM
  • Journal: Proc Natl Acad Sci U S A (1997) 94:4086-4091
  • PubMed ID: 9108109