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Specific ROS Detection%2C DAF-FM

Citations & References

Thymosin ß10 expression driven by the human TERT promoter induces ovarian cancer-specific apoptosis through ROS production.

  • Authors: Kim YC, Kim BG, Lee JH,
  • Journal: PLoS One (2012) 7:e35399-e35399
  • PubMed ID: 22623951

Product FAQ

I am using the reactive oxygen species detection dye, Dihydrorhodamine 123, and when I put the dye in a solution of water and illuminate with UV light, I see a quick change in signal, even without cells. What is happening and how can I stop this?

Answer

You cannot stop it. Any time you illuminate a dye, including this one, photodegradation of the dye will lead to the production of singlet oxygen and free radicals. This in turn causes many ROS indicators, like this one, to become oxidized. If you are testing ROS using UV illumination, all you can do is use this as a baseline and subtract the signal from the sample with cells. The best you can do is reduce the illumination intensity (though this will also reduce the specific signal emission).

Answer Id: E6451

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Citations & References

Glutathione depletion is necessary for apoptosis in lymphoid cells independent of reactive oxygen species formation.

  • Authors: Franco R, Panayiotidis MI, Cidlowski JA,
  • Journal: J Biol Chem (2007) 282:30452-30465
  • PubMed ID: 17724027

Manual / Product Insert

CellROX Oxidative Stress Reagents

Version: 6 May 2012

Citations & References

Distinct mitochondrial retrograde signals control the G1-S cell cycle checkpoint.

  • Authors: Owusu-Ansah E, Yavari A, Mandal S, Banerjee U,
  • Journal: Nat Genet (2008) 40:356-361
  • PubMed ID: 18246068

Citations & References

High glucose-induced oxidative stress and mitochondrial dysfunction in neurons.

  • Authors: Russell JW, Golovoy D, Vincent AM, Mahendru P, Olzmann JA, Mentzer A, Feldman EL
  • Journal: FASEB J (2002) 16:1738-1748
  • PubMed ID: 12409316
Catalog #

Citations & References

Quercetin modifies reactive oxygen levels but exerts only partial protection against oxidative stress within HL-60 cells.

  • Authors: Bestwick CS, Milne L
  • Journal: Biochim Biophys Acta (2001) 1528:49-59
  • PubMed ID: 11514098

Manual / Product Insert

Manual: Reactive Oxygen Species (ROS) Detection Reagents (English)

Version: 01-10-2006
Catalog #

Citations & References

The ability of neonatal and maternal erythrocytes to produce reactive oxygen species in response to oxidative stress.

  • Authors: Kondo M, Itoh S, Kusaka T, Imai T, Isobe K, Onishi S
  • Journal: Early Hum Dev (2002) 66:81-88
  • PubMed ID: 11872312
Catalog #
  • M23800(Discontinued)

Citations & References

Hydrogen-rich saline attenuates radiation-induced male germ cell loss in mice through reducing hydroxyl radicals.

  • Authors: Chuai Y, Gao F, Li B, Zhao L, Qian L, Cao F, Wang L, Sun X, Cui J, Cai J,
  • Journal: Biochem J (2012) 442:49-56
  • PubMed ID: 22077489
Catalog #

Citations & References

Morphological and flow-cytometric analysis of haemin-induced human neutrophil activation: implications for transfusion-related acute lung injury.

  • Authors: Kono M, Saigo K, Takagi Y, Kawauchi S, Wada A, Hashimoto M, Sugimoto T, Takenokuchi M, Morikawa T, Funakoshi K,
  • Journal: Blood Transfus (2013) 11:53-60
  • PubMed ID: 22790260
Catalog #

Citations & References

NADPH oxidases regulate CD44 and hyaluronic acid expression in thrombin-treated vascular smooth muscle cells and in atherosclerosis.

  • Authors: Vendrov AE, Madamanchi NR, Niu XL, Molnar KC, Runge M, Szyndralewiez C, Page P, Runge MS
  • Journal: J Biol Chem 2010 34:26545-26557
  • PubMed ID: 20558727

Citations & References

On the use of fluorescence probes for detecting reactive oxygen and nitrogen species associated with photodynamic therapy.

  • Authors: Price M, Kessel D,
  • Journal: J Biomed Opt (2010) 15:051605-051605
  • PubMed ID: 21054079

Citations & References

Implications of the generation of reactive oxygen species by photoactivated calcein for mitochondrial studies.

  • Authors: Beghetto C, Renken C, Eriksson O, Jori G, Bernardi P, Ricchelli F
  • Journal: Eur J Biochem (2000) 267:5585-5592
  • PubMed ID: 10951218

Product Literature

White Paper: Culture Microbiology Review - Vol. 12, No. 1, March 1991