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CT1410-TS

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Lot # 946515-COA

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What is the purity of the exosomes recovered with the Total Exosome Isolation reagent? Is it similar to ultracentrifugation?

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Answer

The obtained sample contains all exosomes, with insignificant amounts of some other microvesicles, and large protein molecules/complexes that have been co-precipitated (in the case of serum and some of the other body fluids). This purity level works fine for most applications and is balanced by the method benefits which include a fast and simple workflow, no need for special equipment (such as an ultracentrifuge), complete recovery of exosomes, flexibility to work with small sample volumes (e.g., 100 μL), and the capability to process multiple samples in one experiment.

For ultra-pure exosomes from cell culture media, we have available Dynabeads™ magentic beads decorated with anti-CD63 antibodies, which allow recovery of a very clean population of exosomes, following initial purification with the Total Exosome Isolation Reagent (from cell media). Workflow time will increase and the final yield of the exosomes will be lower, but for projects that require an ultra-clean population of CD63-positive exosomes from cell culture media this is the best option.

Dynabeads™ magnetic beads complexed with streptavidin (Cat. No. 10608D) are also available for use with a customer‘s own biotinylated antibodies specific for their exosome sub-population of choice. These products can be used not only for isolation of highly pure exosome sub-populations, but also allow detection of exosomes with flow cytometry, something that has been extremely difficult to achieve due to their small size. We believe that these products are currently the best options for exosome isolation, but as the definition of exosomes solidifies and the demands of the field of microvesicle research become clearer, we can focus on additional products

Answer Id: E7452

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CT1411-TS

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Do the Total Exosome Isolation reagents allow isolation of exosomes from other species besides human?

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Answer

Yes, the reagents can also be used with mouse samples. Presumably, the reagents can also isolate exosomes from samples of any species, but they have only been tested with human and mouse.

Answer Id: E7453

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CT1412-TS

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