Product FAQ

What are the minimum and recommended hardware requirements for GTC 3.0.1?

Answer

Minimum hardware requirements:
-Memory (RAM): 2 GB
-Hard drive: 100 GB*
-Processor: 2.0 GHz Intel Pentium or higher

Recommended hardware requirements:
-Memory (RAM): 4 GB
-Hard drive: 100 GB*
-Processor: 2.5 GHz Intel Quad Core or higher

*Note: The hard drive requirements are for GTC 3.0.1 only; data needs additional space.

Answer Id: E13669

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Product FAQ

What is the difference between the MicroAmp™ Optical Adhesive Covers and the MicroAmp™ Optical Caps? Can they be used interchangeably?

Answer

The MicroAmp™ Optical Adhesive Covers (P/N 4311971) have a higher optical efficiency than the MicroAmp™ Optical Caps, (8 caps/strip) (P/N 4323032). They can be used instead of the MicroAmp™ Optical Caps only when using the MicroAmp™ 96-Well Reaction Plate with Barcode (P/N 4306737, 4309849) or the MicroAmp™ Optical 96-Well Reaction Plate (P/N N801-0560). The Optical Adhesive Covers cannot be used with the MicroAmp™ Optical Tubes.

Answer Id: E1366

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Product FAQ

Is it necessary to uninstall prior versions before installing GTC 3.0.1?

Answer

Previous versions do not need to be uninstalled prior to installing GTC 3.0.1. The GTC 3.0.1 installer will uninstall the older GTC version and then install GTC 3.0.1.

Answer Id: E13670

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Product FAQ

What are the blue particles in the Stainer B solution (Colloidal Blue)?

Answer

The blue particles are called colloids. They are what enables the kit to work effectively.

Just remember to shake the solution well before using to evenly distribute them throughout the bottle.

Answer Id: E3710

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Product FAQ

Can GTC 3.0.1 coexist with older GTC versions on the same computer?

Answer

No, prior GTC versions cannot run on the same computer with GTC 3.0.1.

Answer Id: E13671

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Product FAQ

What are the passing thresholds in GTC 3.0.1 for the Median Absolute Pairwise Difference (MAPD) metric?

Answer

The MAPD passing thresholds for SNP Array 6.0 copy number data are as follows:

-Affymetrix™ Reference: passing MAPD is 0.35 or less (Derived from the HapMap 270 data set)
-External Reference: passing MAPD is 0.35 or less (Chips generated in different lab and with different reagent lots)
-Internal Reference: passing MAPD is 0.30 or less (Chips generated in same lab with same reagent lots)

Answer Id: E13687

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Product FAQ

I am using a cell-free expression system and my protein is forming aggregates. What should I do?

Answer

You can try to reduce the incubation temperature to 25-30 degrees C during protein synthesis. Additionally, a mild detergent can be added (e.g., up to 0.05% Triton-X-100, 0.025% sodium dodecyl maltoside, 0.1% CHAPS, or 0.05% Brij-58) to the reaction and feed buffer. You can also try to add molecular chaperones to the reaction.

Answer Id: E9681

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Product FAQ

Your MembranePro™ Functional Protein Expression Kit/Expi293™ MembranePro™ Expression System does not include a positive control. How do I tell if MembranePro™ particles were formed?

Answer

You can visually tell if MembranePro™ particles formed via a pellet at the bottom of the tube following precipitation. You can also test the function of your MembranePro™ particles via receptor-ligand binding studies.

Answer Id: E9281

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Product FAQ

How many isolations does the Dynabeads™ DNA Direct Blood kit support?

Answer

The Dynabeads™ DNA Direct Blood kit (Cat. No. 63102) supports 100 isolations from 100 μL of blood per sample, sufficient for at least 100 PCR-amplifications. The protocol can be modified to allow isolation from 500 μL blood samples, providing enough template-DNA for 1,000 PCR-amplifications.

Answer Id: E4789

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Product FAQ

I have a copy of Variant Reporter™ Software v.2 and want to know what the computer requirements are.

Answer

The computer requirements are as follows:

Minimum: 2.0 GHz or faster Intel™ processor, 2 GB RAM, 1 GB hard disk space for application
Recommended CPU: Core 2 Duo E8400/3.0 GHz, 4 GB RAM, 1 GB hard disk space
For the operating system, Variant Reporter™ Software v.2 requires Windows™ 7 Professional, 32-bit edition, service pack 1.

Answer Id: E8948

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Product FAQ

Why does a precipitate sometimes form when Colloidal Blue Stainers A and B are mixed together?

Answer

The precipitate is caused by a temporary high concentration of one of the components in a localized area.

Gentle mixing of the solution allows the precipitate to go into solution.

Answer Id: E3711

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Product FAQ

What do antibody pair kits contain, how many samples can I process, and how can I find a list of antibody pair kits by target?

Answer

Antibody pair kits contain capture antibody, detection antibody, recombinant standard and HRP conjugate. Each contains enough reagents to process forty 96-well plates. A list of Antibody Pair Kits (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/elisa/antibody-pair-kits.html) is available by target.

Answer Id: E12615

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Product FAQ

What other software components are installed with GTC 3.0.1?

Answer

The Microsoft .NET 2.0 framework, Java and Visual C++ runtime environments are installed with GTC 3.0.1.

Answer Id: E13672

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Product FAQ

How do you label the FluoSpheres™ microspheres with dye?

Answer

The polystyrene microspheres are swelled in a solvent containing a hydrophobic dye specific to the FluoSpheres™ product. The dye is then able to diffuse into the polystyrene matrix. The beads are then removed from the solvent and dialyzed into an aqueous environment; this reverses the swelling and traps the dye in the polystyrene. Once trapped, the dye is fairly protected from the external environment. The exact protocol and identity of the hydrophobic dyes are proprietary.

Answer Id: E12244

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Product FAQ

Which copy number workflows for the 100K Set/500K Array Set are supported in GTC 3.0.1?

Answer

The following paired and unpaired analysis workflows are supported for the 100K Set/500K Array Set:
-Copy number (CN)
-Loss of heterozygosity (LOH)
-Copy number segment reporting
-Custom region copy number segment reporting

-Paired CN Analysis is used to compare two samples from the same individual to look for copy number differences in different types of tissues.
-Unpaired CN Analysis is used to compare sample files to a set of reference files.

Answer Id: E13688

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