Our all-encompassing range of solutions for western blotting analysis is meticulously designed and tested to meet the highest standards and minimize time and effort. Establish a robust foundation for your western blotting experiments.
Load and go— leverage sequential lateral flow technology with no batteries, shakers, trays, or timers required
Flexible throughput—pick a platform that matches your throughput needs; process 1 mini or midi blot, 2 mini blots, or 6 vertically cut strips using the same or different conditions
Reproducibility—help reduce variability introduced with more hands-on, traditional processing
If you need an everyday solution to analyze a broad range of protein expression levels, an exceptionally sensitive substrate, or a solution for quantitative western blotting, we have the right chemiluminescent substrate for you.
Smooth imaging experience with minimal effort—simple, clean touchscreen interface with logical workflows, complete with a suite of automated features
The core imaging capabilities that you need—fluorescent blot, chemiluminescent blot, protein gel, and DNA gel imaging
The special application capabilities that you want—image membrane stains, 2D strips, TLC plates, colorimetric blots, colony plates, perform total protein normalization and more
Load and go— leverage sequential lateral flow technology with no batteries, shakers, trays, or timers required
Flexible throughput—pick a platform that matches your throughput needs; process 1 mini or midi blot, 2 mini blots, or 6 vertically cut strips using the same or different conditions
Reproducibility—help reduce variability introduced with more hands-on, traditional processing
If you need an everyday solution to analyze a broad range of protein expression levels, an exceptionally sensitive substrate, or a solution for quantitative western blotting, we have the right chemiluminescent substrate for you.
Smooth imaging experience with minimal effort—simple, clean touchscreen interface with logical workflows, complete with a suite of automated features
The core imaging capabilities that you need—fluorescent blot, chemiluminescent blot, protein gel, and DNA gel imaging
The special application capabilities that you want—image membrane stains, 2D strips, TLC plates, colorimetric blots, colony plates, perform total protein normalization and more
Help improve the success rate of your western blotting experiments by starting with carefully curated product combinations. Designed to work easily together to help improve lab efficiency and support your research.
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Total protein normalization (TPN) for quantitative western blotting analysis offers several advantages over traditional housekeeping protein-based (HKP) normalization. One optimal protein labeling reagent for TPN is the No-Stain Protein Labeling Reagent. TPN using No-Stain Labeling Reagent exhibits good linearity of response within the concentration range typically investigated (1–80 µg of protein load). The wider dynamic range prevents the loss of valuable quantitative data due to signal saturation, a common occurrence with HKP normalization. TPN using No-Stain Labeling Reagent simplifies experimental design, helping save time and resources. Unlike HKP normalization, there is no need to verify that the expression of housekeeping proteins remains unaffected by the treatment. No-Stain Labeling Reagent is particularly well-suited for TPN due to its rapid labeling protocol, and compatibility with downstream transfer and immunoblotting. It enables sensitive and stable signal detection down to 20 ng of proteins, allowing for accurate quantitation of protein expression levels.
Multiplexed western blotting is a powerful technique that offers several advantages over traditional western blotting. Its ability to simultaneously detect and quantify multiple proteins within a single sample helps increase efficiency, improves accuracy, reduces costs, and enhances data quality. It is advantageous when investigating complex biological pathways involving proteins with diverse molecular weights. The simultaneous detection of multiple protein targets in one experiment reduces experimental variability and is an excellent choice when the sample volume is limited or when analyzing large sample sets. Fluorescent western blotting requires careful planning and experiment design. Selecting the right products from the start reduces optimization and troubleshooting time and helps ensure the success of your experiment.
Fluorescence western blotting—Two-part application note
If you are new to fluorescence-based western blotting or if you want to maximize the outcome of your multiplexing experiments, this two-part application note includes best practices, troubleshooting tips, and easy-to-apply protocols.
Protein Electrophoresis and Western Blotting Handbooks
The Protein Gel Electrophoresis and Western Blotting Handbooks are comprehensive, easy-to-use guides covering every procedural step from sample prep to imaging and data analysis.
Whether you are new to western blotting, or an experienced researcher wanting to confirm your knowledge, access our educational tips and resource materials to help with every aspect of western blot analysis.
Western blotting tips, tricks and troubleshooting guide
No bands, too many bands, blotchy blot? This guide shares valuable insights to help solve and avoid these and other common pitfalls researchers encounter when performing western blotting.
Try before you buy. Request one of our top performing western blotting and protein biology products to find the right solutions for your unique protein. Simply select your product of interest and fill out the form.