Protein detection and analysis—western blotting

Our all-encompassing range of solutions for western blotting analysis is meticulously designed and tested to meet the highest standards and minimize time and effort. Establish a robust foundation for your western blotting experiments.


Innovative western blotting solutions

Boost your productivity with western blotting tools designed for efficient high-quality data generation. 

 

 

 

 


Product combinations for western blot success

Help improve the success rate of your western blotting experiments by starting with carefully curated product combinations. Designed to work easily together to help improve lab efficiency and support your research.

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    Western blot of high molecular weight proteins

    Begin with excellent protein separation and efficient protein transfer with NuPAGE Tris-Acetate Gels and iBlot 3 Western Blot Transfer System.

    Application note: Optimizing western blot transfer for high and low molecular weight proteins

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        Run high performance protein gels in Bio-Rad’s Tank

        Get sharp, straight bands no matter your protein molecular weight with Tetra Electrophoresis Cores and Invitrogen Precast Protein gels.

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            Easy total protein normalization

            Streamline the quantitative western blotting workflow and help overcome the limitations of housekeeping protein-based normalization with iBright FL1500 Imaging System and No-Stain Protein Labeling Reagent.

            Application note: Easy and accurate normalization of western blot data

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                Enhanced detection of low abundance proteins or dilute samples

                Double your sample loading volume and help improve the sensitivity of chemiluminescent detection with WedgeWell format protein gels and SuperSignal West Atto Ultimate Sensitivity Substrate.



                Everyday essentials for western blotting

                Limit the variables in your experiments by using dependable products from one trusted source.


                Total protein normalization

                Total protein normalization (TPN) for quantitative western blotting analysis offers several advantages over traditional housekeeping protein-based (HKP) normalization. One optimal protein labeling reagent for TPN is the No-Stain Protein Labeling Reagent. TPN using No-Stain Labeling Reagent exhibits good linearity of response within the concentration range typically investigated (1–80 µg of protein load). The wider dynamic range prevents the loss of valuable quantitative data due to signal saturation, a common occurrence with HKP normalization. TPN using No-Stain Labeling Reagent simplifies experimental design, helping save time and resources. Unlike HKP normalization, there is no need to verify that the expression of housekeeping proteins remains unaffected by the treatment. No-Stain Labeling Reagent is particularly well-suited for TPN due to its rapid labeling protocol, and compatibility with downstream transfer and immunoblotting. It enables sensitive and stable signal detection down to 20 ng of proteins, allowing for accurate quantitation of protein expression levels.

                Learn more about TPN with No-Stain Labeling Reagent


                Multiplexed fluorescent western blotting

                Multiplexed western blotting is a powerful technique that offers several advantages over traditional western blotting. Its ability to simultaneously detect and quantify multiple proteins within a single sample helps increase efficiency, improves accuracy, reduces costs, and enhances data quality. It is advantageous when investigating complex biological pathways involving proteins with diverse molecular weights. The simultaneous detection of multiple protein targets in one experiment reduces experimental variability and is an excellent choice when the sample volume is limited or when analyzing large sample sets. Fluorescent western blotting requires careful planning and experiment design. Selecting the right products from the start reduces optimization and troubleshooting time and helps ensure the success of your experiment.

                Design your next multiplex western blotting experiment

                Fluorescence western blotting—Two-part application note

                If you are new to fluorescence-based western blotting or if you want to maximize the outcome of your multiplexing experiments, this two-part application note includes best practices, troubleshooting tips, and easy-to-apply protocols.

                Fill out the form to get access

                Protein Electrophoresis and Western Blotting Handbooks

                The Protein Gel Electrophoresis and Western Blotting Handbooks are comprehensive, easy-to-use guides covering every procedural step from sample prep to imaging and data analysis.

                Get the handbooks


                Western blot learning and troubleshooting

                Whether you are new to western blotting, or an experienced researcher wanting to confirm your knowledge, access our educational tips and resource materials to help with every aspect of western blot analysis.

                Access western blotting learning center

                Western blotting tips, tricks and troubleshooting guide

                No bands, too many bands, blotchy blot? This guide shares valuable insights to help solve and avoid these and other common pitfalls researchers encounter when performing western blotting.

                Download your copy of the guide

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                Videos
                Discover the new ways to do western blotting with our how-to videos.
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                Protocols
                Find recipes for buffers, stock solutions, and general western blot protocols.
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                Foundation Library
                Tech tips, application notes, handbooks and more.
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                Troubleshooting
                Overcome western blot difficulties with our troubleshooting guidance.

                Protein Research Essentials Sampling Program

                Try before you buy. Request one of our top performing western blotting and protein biology products to find the right solutions for your unique protein. Simply select your product of interest and fill out the form.

                Request samples now

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                For Research Use Only. Not for use in diagnostic procedures.