Researchers need antibodies that bind to the right target and function effectively in their intended applications. Not having the right antibody can have far-reaching effects that include:
- Lost time and money
- Compromised experimental results
- Lack of experimental reproducibility
The Four Pillars of Invitrogen antibody specificity validation* were developed to help minimize poor antibody performance. In addition to our rigorous functional application validation testing, Invitrogen antibodies are assessed using advanced validation methods that go beyond our standard testing. Including these additional steps provide further assurance that our antibodies will bind to their intended targets. The four pillars include these distinct testing methods:
- Immunoprecipitation and mass spectrometry (IP-MS)
- Genetic modification
- Independent antibody verification (IAV)
- Biological verification
This elearning course was developed to help you better understand the principles behind these specificity testing methods so that you know what questions to ask when choosing antibodies for your research.
Each of the four modules is interactive, and you will have opportunities to test your knowledge along the way. Each module takes approximately 30 minutes to view. The tabs below provide more detailed descriptions and the learning objectives for each module.
* The use or any variation of the word “validation” refers only to research use antibodies that were subject to functional testing to confirm that the antibody can be used with the research techniques indicated. It does not ensure that the product, or products were validated for clinical or diagnostic use.