Thank you for creating beautiful cell images with us.

Congratulations to the following winners:

1st Prize Winner – Jaron Liu (Institute of Medical Biology, Singapore)
2nd Prize Winner – Chan Jia Pei (Duke-NUS Medical School, Singapore)
3rd Prize Winner – Jonathan Aow ( National University of Singapore, Singapore)

Join us at the Cell-ebrate Science Prize Giving Ceremony & Cell Analysis Roadshow on 7th March 2017, Matrix, Biopolis.

Seeking beautiful science cell images

  • To provide a platform for scientists in the Singapore, Southeast Asia & Taiwan region to showcase their research using our Molecular Probes™ reagents
  • Educate customer to discover the use of Molecular Probes™ brand fluorescent products by leveraging our customer’s experience, expertise and brand
  • To combine science & art to create “beautiful science” images
  • *The competition is opened to participants from Singapore, Southeast Asia & Taiwan.

Graham Wright

Head, Microscopy Unit
Institute of Medical Biology, A*STAR
Singapore

Graham Wright is the Head, Microscopy Unit at A*STAR’s Institute of Medical Biology. He has a PhD in cell biology from The University of Edinburgh, UK and extensive experience of applying light microscopy techniques to address cell and developmental biology research questions. Through collaborative projects, utilising advanced microscopes, he has co-authored numerous papers in high-impact journals. The IMB Microscopy Unit which Graham manages is a core technology platform, comprising over 20 instruments, providing light and electron microscopy equipment along with image processing and analysis software and the expertise to facilitate the institute’s research.

Magnus Persmark

Senior Product Manager
Thermo Fisher Scientific – Cell Analysis
Eugene, Oregon, USA

Magnus Persmark is a Senior Product Manager with the Cell Analysis unit of Thermo Fisher Scientific.  His current responsibilities include the EVOS™ microscope portfolio and he previously managed various product product families from Molecular Probes with applications in the cellular function, proliferation, and cell health areas.

Magnus received his PhD in Biotechnology from the Lund Institute of Technology, spending four years on research at the Department of Biochemistry at UC Berkeley.  He conducted postdoctoral studies at Vanderbilt University and UNC Chapel Hill before taking a position in Biopharmaceutical Development at GlaxoWellcome.  He joined the company in 2005 and has more than 25 years of experience in cell-based research.

Michael S. Janes, M.S.

Senior Manager, Research & Development
Thermo Fisher Scientific - Biosciences Division
Eugene, Oregon, USA

Mike received his M.S. in 1996 from Bowling Green State University in Bowling Green, Ohio, USA, where his graduate work focused on mitochondrial biology and comparative biochemistry between parasitic helminths and their mammalian hosts. Mike continued his training as a cell biologist and microscopist at National Jewish Medical Center in Denver, Colorado where he developed fluorescence-based assays for the study of inflammation and apoptosis in respiratory diseases until 2000. He has been a research scientist at Thermo Fisher Scientific developing Molecular Probes reagents and assays for fluorescence microscopy for sixteen years.

Rules and regulations

Eligibility

  1. Participation is open to people aged 21 years and above in Singapore, Southeast Asia & Taiwan. Healthcare professionals and government officials may not participate in this competition. It is the responsibility of the program participant to determine his or her eligibility based on governing law and the policies and practices of his or her institution or employer or industry codes of conduct. Employees of Thermo Fisher Scientific and its affiliates, as well as their family and household members, are not eligible to participate.
  2. An image that has won other competitions is not eligible to win this contest.
  3. The submitted entry must be the entrant’s own work or the work of their institution / employer and must have been undertaken using Molecular probes reagents solely from Thermo Fisher Scientific. The entrant would require a completed and signed official entry form from his/her institution/ employer (Principal Investigator) in order to enter the contest using the images that are being submitted.
  4. Images can be taken using any microscope. All types of light microscopy and specimens are acceptable. Electron and any other microscopes that do not capture imagery using optical light technology are not permitted.
  5. No purchase is necessary to enter or win.

How to Enter

  1. Images must be submitted online as follows:
    1. Visit www.thermofisher.com/cellimagingcontest and provide the requested contact information together with your submission, which should consist of the following elements:
      1. up to three images of your research
      2. a short abstract describing your research (200 words)
      3. Thermo Fisher Scientific reagents that are used in your research
    2. Maximum file size is 100MB, and it is recommended the file size be no smaller than 3MB. Files must be in jpeg or pdf format. (Please submit the highest quality image with regard to file size, bit depth, and resolution.)

Deadline for Submission

  1. The Cell-ebrate Science Imaging Contest begins 22th June 2016. Contest submissions must be received no later than 6:00 p.m. Singapore Time, 30th October 2016.
  2. Please send enquires to events.sea@thermofisher.com.

Judging Criteria

  1. Winners will be selected by a panel of 3 qualified judges comprised of Sponsor’s employee and experts with backgrounds in cell imaging.
  2. Criteria for judging the winning entries will be based on:
    1. Originality of the image (35%),
    2. Technical proficiency (35%)
    3. Short abstract submitted on the project (30%)
  3. All judging criteria will be determined by the Sponsor’s employee and experts in cell imaging except the number of votes for the images in the link provided on our website which will be open to the public. Only one vote per person will be permitted. The scores given by all the three judges will be averaged and this makes up 90% of the total score. The judges’ decision will be final.

Winners Selection

  1. The top 3 entries will be selected by approximately November 2016 from all eligible entries received. Each winner will be notified by email, telephone, or mail within approximately 14 days after the drawing date. If a winner is unreachable or does not make direct contact with the Sponsor by the date specified in the notification, or fails to accept the prize, the prize will be forfeited in its entirety and the submission that garnered the next highest scores will be selected as a winner. The winner must execute and return the affidavit of eligibility and a liability release within 14 days of the date of notification. If a winner fails to do so, the prize will be forfeited in its entirety and an alternate winner will be selected from the remaining eligible entries. The winner must warrant that he or she is not a healthcare profession, nor prohibited by employment, contract, or law from entering the contest or accepting a prize from the Sponsor.

Prizes

  1. Prizes will be awarded as follow:
    1. Entry that wins the first prize will receive $1000 Thermo Fisher Scientific credits that can be used to purchase a list of qualifying products in a single purchase order. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Winning image will be featured on the Bioprobes magazine. Winner will receive an iPad Pro 32GB 9.7inch, and a sponsored Christmas festive buffet for 20 pax. The total estimated value of the entire prize is $2200 SGD.
    2. The second prize winner will receive a $500 Thermo Fisher Scientific credits that can be used to purchase a list of qualifying products in a single purchase order. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Winner will get to receive an iPad mini 4 16 GB. The estimated value of the entire prize is $1100 SGD.
    3. The third prize winner will receive a $300 Thermo Fisher Scientific vouchers that can be used to purchase a list of qualifying products in a single purchase order. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Winner will get to receive a Sony HDR-AS50 Action Camera. The estimated value of the entire prize is $600 SGD.
    4. No cash or other substitutions are permitted, except by the Sponsor, which reserves the right to substitute the prize with one of equal or greater value. No refunds or credits are allowed. The prize is non-transferable. All taxes on the prize and reporting thereof are the sole responsibility of the Winner.

Disclaimers

  1. All entries and their content become the property of the Sponsor and will not be returned. This Contest shall be governed by and construed in accordance with the laws of the Singapore without reference to its choice-of-law doctrines. By entering the Contest, you agree to receive further information from the Sponsor in accordance with the Sponsor’s privacy policy, which can be found here.
  2. In no event shall the sponsor be liable, whether in contract, tort, warranty, or under any statute or on any other basis for special, incidental, indirect, punitive, multiple or consequential damages sustained by any winner or any other person or entity arising out of the sponsor’s performance or failure to perform its obligations relating to the award of the prize or performance of services. The possession or use of any product, or the performance by the sponsor of any services, whether or not foreseeable and whether or not the sponsor is advised of the possibility of such damages, including without limitation damages arising from or related to loss of use, loss of data, downtime, or for loss of revenue, profits, goodwill, or business or other financial loss.
  3. Winning images will only be used in connection with the contest and accompanying publicity and will include appropriate photo credits.
  4. If the submitted images have more than one individual to be credited, please provide the names on the entry form. Individual who enter the contest also warrants that they have the right to enter the image on the other parties behalf and holds Thermo Fisher Scientific harmless in any disputes that arise thereof.
  5. Failure to comply with and accept all of the preceding rules will disqualify any entry and Thermo Fisher Scientific reserves the right to disqualify any entrant for any reason at Thermo Fisher Scientific’s sole discretion.
  6. Thermo Fisher Scientific reserves the right to change or modify the rules at any time without notice.

Trademark Attribution Section

  1. Apple, Facebook and Sony are not a sponsor or a participant of this contest. This contest is no way sponsored or administered by or associated with Apple, Facebook and Sony. You understand that you are providing your information to Thermo Fisher Scientific and not to the above companies mentioned. You may view Thermo Fisher Scientific’s privacy policy here

By entering the contest, each participant unconditionally accepts and agrees to comply with and abide by these rules and the decisions of the Sponsor, which shall be final and binding in all respects.

Consent form Download Now

Image release form Download Now



Grand Prize

  • iPad Pro
  • $1000 prize credits for purchase of Molecular Probes™ reagents
  • Image published on our Bioprobes magazine
  • Celebration Buffet for 20 pax




2nd Prize

  • iPad mini 4
  • $500 prize credits for purchase of Molecular Probes™ reagents




3rd Prize

  • Sony HDR-AS50 Action Camera
  • $300 prize credits for purchase of Molecular Probes™ reagents

If you would like to get in touch, please email to the following contacts:

For Singapore & Southeast Asia participants – events.sea@thermofisher.com

For Taiwan participants – marketing.taiwan@thermofisher.com

We are here to help! :)

Thanks,
Cell-ebrate Science Organising Committee

Induction of filopodia-like protrusions in N1E-115 neuroblastoma cells

Investigation of the nanoscale architecture of filopodia using state-of-the-art super-resolution technique. Acquired on DeltaVision OMX 3D-SIM, 100X 1.4NA lens, 3um stack, 125nm per stack, maximum projection, image pixel size 37.5nm. Stained with Alexa-568 phalloidin.

Lung cancer cell A549

A549 stain with phalloidin and beta-catenin antibody

Hepatocytes 96 hours after partial hepatectomy

Hepatocytes were isolated from livers of mus musculus 96 hours after 70% partial hepatectomy. Alexa Fluor 488 phalloidin was used to stain the cytoskeleton, Mitotracker Red CMXRos was used to stain the mitochondria and Hoechst 33342 (trihydrochloride, trihydrate) was used to stain the nucleus.

The Kraken cell

Insert your abstract here. (No more than 200 words.) The proliferating cancer cell was transformed upon stress stimulation. it now looked like a "kraken" cell, showing neuronal-like dendrites. The cyan color indicated alpha-tubulin. The pink color indicated EGFP. The red color indicated a cytosolic protein which was transported among dendrites. The Alexa Flour dye conjugated secondary antibodies (Alexa Flour 647 conjugated Goat anti-mouse IgG; Alexa Flour 555 donkey anti-rabbit IgG) were from Molecular probes. Blue color indicated Hoechst 33342 dye stained nucleus.

Psychedelic cerebrum

A coronal section of a mouse brain is stained with different markers - Myelin in green (Alexa Fluor 488), axons in red (Alexa Fluor 555), microglia (Alexa Fluor 633) in magenta and nuclei in blue (Hoescht 33342). One can appreciate the structure of cortex, hippocampus, striatum, thalamus and hypothalamus in this section.

Eat to Live

The spontaneously transformed immortal keratinocyte (HaCaT) cell line has been widely used for various applications. Here, we adopted the use of this cell line to study the consequence of bacteria-host pathogen interactions. This particular islet took on the appearance of a tortoise, anticipating its next meal. HaCaTs were stained with the following probes – Hoechst 33342 (blue), α-tublin Alexa Fluor 488 (green) and Alexa Fluor 568 phalloidin (red).

Stem cell differentiation: Neural progenitor cells

Human embryonic stem cell is differentiated into neural progenitor cell using neural differentiation media (N2B27 media with small molecules/growth factors). The nucleus is stained with DAPI while the intermediate filaments are stained with Alexa Fluor® 647 dye.

GFP-expressing primary hippocampal neuron

The hippocampus is essential for memory formation, and hippocampal dysfunction and degeneration underlies memory impairment observed in dementias such as Alzheimer's disease. Hippocampal neurons in culture replicate key in vivo neuronal features, including the formation of dendritic spines, and also exhibit synaptic plasticity, the molecular correlate of learning and memory. Because neurons can be transfected with a variety of fluorescently tagged markers (such as GFP in this instance) or stained easily with antibodies, they have proven invaluable for probing gene function in neuroscience. The single layer culture is also particularly amenable and advantageous to standard confocal light microscopy.

Primary hippocampal neurons were transfected with GFP at 15 days in vitro (DIV) and fixed and stained 2 days later. The somatodendritic compartment was stained using an antibody against Map2A/B and an Alexa-647 secondary antibody (shown here in red; neighboring non-transfected neurons are therefore also visible). Axons were stained using an antibody against NF-L and an Alexa-405 secondary antibody (shown here in blue). Images were acquired on a Zeiss LSM700 confocal microscope with a 63x objective.

Actin cytoskeleton organization of A549

This image shown the actin cytoskeleton organization of A549 cells (non small cell lung cancer) Actin cytoskeleton were stained by Alexa Fluor488® phalloidin shown in green color and nucleus were stained by DAPI shown in blue color. Image were acquired on a zeiss LSM700 confocal microscope.

Keratinocyte piglet

This picture is a fluorescent microscope image of human primary epidermal keratinocytes stained with nuclear stain (NucBlue® Live ReadyProbes® Reagent). The keratinocyte cells grow in sheets, here it resembles the shape of a piglet. The picture was captured with 20X magnification.

Microfluidic Vessel on-a-Chip

Human Umbilical Vein Endothelial Cells (HUVEC) cells are grown in a microfluidic chip to mimic a blood vessel endothelial layer (Vessel on-a-chip). Cells are stained and imaged as green for VE-Cadherin using Alexa Fluor 488 goat anti-rabbit IgG (H+L) secondary antibody (A11034), as red for F-actin using Alexa Fluor 568 Phalloidin (A12380), and as blue for nucleus using Hoechst 33342 dye (H3570). Image is captured at 40x magnification with an inverted fluorescence microscope (Nikon Eclipse Ti).

Where life begins

Mouse eggs

Confocals Olympus FV1000 Inverted

Alexa fluor Phalloidin 568
Alphatubulin antibody, Alexa fluor secondary 488 Dapi 405

Pores for thought

The nucleus of a HeLa cell, recognized by an antibody specific to the nuclear pore complex followed by staining with Alexa 647 secondary antibody (magenta), and visualized by conventional optical microscopy (left) and dSTORM super-resolution microscopy (right).

While individual nuclear pore complexes are blurry in conventional microscopy, they can be easily resolved using dSTORM with approximately 10 nm precision.

Differentiation of human embryonic stem cells to neuroepithelial cells

The images attached were captured during characterization of my samples as a part of my Ph.D. research work based on the application of biomechanical forces for efficient differentiation of human embryonic stem cells to neuroepithelial cells. Neuroepithelial cells are an important class of neuro progenitor cells required for formation of the brain and spinal cord in any organism. During embryonic development, the generation of these neuroepithelial cells occurs under controlled biochemical and biomechanical forces and in my project I aim to generate similar physiological conditions for efficient generation of neuroepithelial cells. These cells can then be used for generation and modeling of human mini brain organoids.
Image 1. Confocal immunofluorescent images of human embryonic stem cells undergoing epithelial to mesenchymal transition on neural induction. The cells are labeled with rabbit anti-Nestin antibody and later tagged with Donkey anti-Rabbit IgG (H+L) Secondary Antibody Alexa Fluor® 647 conjugate (Magenta), mouse anti-Sox2 and later tagged with Donkey anti-Mouse IgG (H+L) Secondary Antibody Alexa Fluor® 555 conjugate (Red) and Alexa Fluor® 488 Phalloidin (Green).