- iPad Pro
- $1000 prize credits for purchase of Molecular Probes™ reagents
- Image published on our Bioprobes magazine
- Celebration Buffet for 20 pax
1st Prize Winner – Jaron Liu (Institute of Medical Biology, Singapore)
2nd Prize Winner – Chan Jia Pei (Duke-NUS Medical School, Singapore)
3rd Prize Winner – Jonathan Aow ( National University of Singapore, Singapore)
Join us at the Cell-ebrate Science Prize Giving Ceremony & Cell Analysis Roadshow on 7th March 2017, Matrix, Biopolis.
Head, Microscopy Unit
Graham Wright is the Head, Microscopy Unit at A*STAR’s Institute of Medical Biology. He has a PhD in cell biology from The University of Edinburgh, UK and extensive experience of applying light microscopy techniques to address cell and developmental biology research questions. Through collaborative projects, utilising advanced microscopes, he has co-authored numerous papers in high-impact journals. The IMB Microscopy Unit which Graham manages is a core technology platform, comprising over 20 instruments, providing light and electron microscopy equipment along with image processing and analysis software and the expertise to facilitate the institute’s research.
Senior Product Manager
Magnus Persmark is a Senior Product Manager with the Cell Analysis unit of Thermo Fisher Scientific. His current responsibilities include the EVOS™ microscope portfolio and he previously managed various product product families from Molecular Probes with applications in the cellular function, proliferation, and cell health areas.
Magnus received his PhD in Biotechnology from the Lund Institute of Technology, spending four years on research at the Department of Biochemistry at UC Berkeley. He conducted postdoctoral studies at Vanderbilt University and UNC Chapel Hill before taking a position in Biopharmaceutical Development at GlaxoWellcome. He joined the company in 2005 and has more than 25 years of experience in cell-based research.
Michael S. Janes, M.S.
Senior Manager, Research & Development
Mike received his M.S. in 1996 from Bowling Green State University in Bowling Green, Ohio, USA, where his graduate work focused on mitochondrial biology and comparative biochemistry between parasitic helminths and their mammalian hosts. Mike continued his training as a cell biologist and microscopist at National Jewish Medical Center in Denver, Colorado where he developed fluorescence-based assays for the study of inflammation and apoptosis in respiratory diseases until 2000. He has been a research scientist at Thermo Fisher Scientific developing Molecular Probes reagents and assays for fluorescence microscopy for sixteen years.
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Investigation of the nanoscale architecture of filopodia using state-of-the-art super-resolution technique. Acquired on DeltaVision OMX 3D-SIM, 100X 1.4NA lens, 3um stack, 125nm per stack, maximum projection, image pixel size 37.5nm. Stained with Alexa-568 phalloidin.
A549 stain with phalloidin and beta-catenin antibody
Hepatocytes were isolated from livers of mus musculus 96 hours after 70% partial hepatectomy. Alexa Fluor 488 phalloidin was used to stain the cytoskeleton, Mitotracker Red CMXRos was used to stain the mitochondria and Hoechst 33342 (trihydrochloride, trihydrate) was used to stain the nucleus.
Insert your abstract here. (No more than 200 words.) The proliferating cancer cell was transformed upon stress stimulation. it now looked like a "kraken" cell, showing neuronal-like dendrites. The cyan color indicated alpha-tubulin. The pink color indicated EGFP. The red color indicated a cytosolic protein which was transported among dendrites. The Alexa Flour dye conjugated secondary antibodies (Alexa Flour 647 conjugated Goat anti-mouse IgG; Alexa Flour 555 donkey anti-rabbit IgG) were from Molecular probes. Blue color indicated Hoechst 33342 dye stained nucleus.
A coronal section of a mouse brain is stained with different markers - Myelin in green (Alexa Fluor 488), axons in red (Alexa Fluor 555), microglia (Alexa Fluor 633) in magenta and nuclei in blue (Hoescht 33342). One can appreciate the structure of cortex, hippocampus, striatum, thalamus and hypothalamus in this section.
The spontaneously transformed immortal keratinocyte (HaCaT) cell line has been widely used for various applications. Here, we adopted the use of this cell line to study the consequence of bacteria-host pathogen interactions. This particular islet took on the appearance of a tortoise, anticipating its next meal. HaCaTs were stained with the following probes – Hoechst 33342 (blue), α-tublin Alexa Fluor 488 (green) and Alexa Fluor 568 phalloidin (red).
Human embryonic stem cell is differentiated into neural progenitor cell using neural differentiation media (N2B27 media with small molecules/growth factors). The nucleus is stained with DAPI while the intermediate filaments are stained with Alexa Fluor® 647 dye.
The hippocampus is essential for memory formation, and hippocampal dysfunction and degeneration underlies memory impairment observed in dementias such as Alzheimer's disease. Hippocampal neurons in culture replicate key in vivo neuronal features, including the formation of dendritic spines, and also exhibit synaptic plasticity, the molecular correlate of learning and memory. Because neurons can be transfected with a variety of fluorescently tagged markers (such as GFP in this instance) or stained easily with antibodies, they have proven invaluable for probing gene function in neuroscience. The single layer culture is also particularly amenable and advantageous to standard confocal light microscopy.
Primary hippocampal neurons were transfected with GFP at 15 days in vitro (DIV) and fixed and stained 2 days later. The somatodendritic compartment was stained using an antibody against Map2A/B and an Alexa-647 secondary antibody (shown here in red; neighboring non-transfected neurons are therefore also visible). Axons were stained using an antibody against NF-L and an Alexa-405 secondary antibody (shown here in blue). Images were acquired on a Zeiss LSM700 confocal microscope with a 63x objective.
This image shown the actin cytoskeleton organization of A549 cells (non small cell lung cancer) Actin cytoskeleton were stained by Alexa Fluor488® phalloidin shown in green color and nucleus were stained by DAPI shown in blue color. Image were acquired on a zeiss LSM700 confocal microscope.
This picture is a fluorescent microscope image of human primary epidermal keratinocytes stained with nuclear stain (NucBlue® Live ReadyProbes® Reagent). The keratinocyte cells grow in sheets, here it resembles the shape of a piglet. The picture was captured with 20X magnification.
Human Umbilical Vein Endothelial Cells (HUVEC) cells are grown in a microfluidic chip to mimic a blood vessel endothelial layer (Vessel on-a-chip). Cells are stained and imaged as green for VE-Cadherin using Alexa Fluor 488 goat anti-rabbit IgG (H+L) secondary antibody (A11034), as red for F-actin using Alexa Fluor 568 Phalloidin (A12380), and as blue for nucleus using Hoechst 33342 dye (H3570). Image is captured at 40x magnification with an inverted fluorescence microscope (Nikon Eclipse Ti).
Confocals Olympus FV1000 Inverted
Alexa fluor Phalloidin 568
Alphatubulin antibody, Alexa fluor secondary 488 Dapi 405
The nucleus of a HeLa cell, recognized by an antibody specific to the nuclear pore complex followed by staining with Alexa 647 secondary antibody (magenta), and visualized by conventional optical microscopy (left) and dSTORM super-resolution microscopy (right).
While individual nuclear pore complexes are blurry in conventional microscopy, they can be easily resolved using dSTORM with approximately 10 nm precision.
The images attached were captured during characterization of my samples as a part of my Ph.D. research work based on the application of biomechanical forces for efficient differentiation of human embryonic stem cells to neuroepithelial cells. Neuroepithelial cells are an important class of neuro progenitor cells required for formation of the brain and spinal cord in any organism. During embryonic development, the generation of these neuroepithelial cells occurs under controlled biochemical and biomechanical forces and in my project I aim to generate similar physiological conditions for efficient generation of neuroepithelial cells. These cells can then be used for generation and modeling of human mini brain organoids.
Image 1. Confocal immunofluorescent images of human embryonic stem cells undergoing epithelial to mesenchymal transition on neural induction. The cells are labeled with rabbit anti-Nestin antibody and later tagged with Donkey anti-Rabbit IgG (H+L) Secondary Antibody Alexa Fluor® 647 conjugate (Magenta), mouse anti-Sox2 and later tagged with Donkey anti-Mouse IgG (H+L) Secondary Antibody Alexa Fluor® 555 conjugate (Red) and Alexa Fluor® 488 Phalloidin (Green).