Workflow for ready-to-transfect format

This workflow combines GeneArt® CRISPR Nuclease mRNA and GeneArt® U6 Strings™ DNA. In this example, Cas9 mRNA is cotransfected with three GeneArt® U6 Strings™ DNA, which allows for multiple gene edits within one transfection. GeneArt® U6 Strings™ DNA are synthetic 500 bp DNA expression cassettes that contain a U6 promoter and that can be directly transfected into the cell with Cas9 mRNA. The U6 promoter drives expression of target-specific gRNA that forms a complex with the Cas9 protein generated from GeneArt® CRISPR Nuclease mRNA. The use of the U6 promoter (a human Pol III promoter) ensures high expression of gRNA. We recommend Lipofectamine® 2000 Transfection Reagent for high-efficiency delivery of GeneArt® CRISPR Nuclease mRNA and GeneArt® U6 Strings™ DNA.

This workflow combines GeneArt® CRISPR Nuclease mRNA and GeneArt® U6 Strings™ DNA. In this example, Cas9 mRNA is cotransfected with three GeneArt® U6 Strings™ DNA, which allows for multiple gene edits within one transfection. GeneArt® U6 Strings™ DNA are synthetic 500 bp DNA expression cassettes that contain a U6 promoter and that can be directly transfected into the cell with Cas9 mRNA. The U6 promoter drives expression of target-specific gRNA that forms a complex with the Cas9 protein generated from GeneArt® CRISPR Nuclease mRNA. The use of the U6 promoter (a human Pol III promoter) ensures high expression of gRNA. We recommend Lipofectamine® 2000 Transfection Reagent for high-efficiency delivery of GeneArt® CRISPR Nuclease mRNA and GeneArt® U6 Strings™ DNA.

Related Products

Related Images

CD335 (NKp46) Antibody (63335182) in RE Go ›

CD223 (LAG-3) Antibody (56223942) in TM Go ›