What is the advantage of using EZ-Link HPDP-Biotin over maleimide- or iodoacetyl-containing biotinylation reagents?
Both maleimide- and iodoacetyl-containing biotinylation reagents react with sulfhydryl (-SH) groups to form stable thioether bonds that are not cleavable. EZ-Link HPDP-Biotin on the other hand is a pyridyldithiol-biotin compound that reacts with -SH groups in near-neutral buffers to form reversible disulfide bonds. Because the disulfide group can be cleaved using DTT or other reducing agents, HPDP-Biotin is useful for labeling and affinity-purification applications that require recovery of the original, unmodified molecule.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.