eBioscience™ Fixable Viability Dye eFluor™ 780, 100 Tests - FAQs

View additional product information for eBioscience™ Fixable Viability Dye eFluor™ 780 - FAQs (65-0865-14, 65-0865-18)

2 product FAQs found

How do I discriminate between live cells and dead cells when doing a flow cytometry analysis?

Discrimination between viable and non-viable cells can be carried out with the use of the 7-AAD Viability Dye (Cat. No. 00-6993-50) or Propidium Iodide (PI) Staining Solution (Cat. No. 00-6990-50). The 7-AAD or PI will mark the non-viable cells by binding to the nuclei of those cells. The nucleic acid of viable cells will not be accessible to the dye and will not be stained. When analyzing the data collected, gate out all cells stained with the viability dye. When staining for intracellular proteins, use the Fixable Viability Dye eFluor 455UV, 450, 520, 660, or 780.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Fixable Viability Dyes be used with the eBioscience BrdU Staining kits for Flow Cytometry?

Yes, a Fixable Viability Dye can be added to samples after BrdU labeling and before fixation with the BrdU Staining Buffer. See the protocol for additional information.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.