Ampicillin - FAQs

How can I prepare LB-ampicillin using Ampicillin (Cat. No. J60977)?

We recommend the following the instructions to prepare LB-ampicillin:

1. Dissolve the following in 500 mL dH2O:
• 5g tryptone
• 2.5g yeast extract
• 5.0g NaCl
• 7.5g agar
OR
• 20g pre-mixed LB agar powder
2. Boil the solution on a hot plate while stirring for 1-2 minutes.
3. Autoclave the solution for 20 minutes and let cool to 50-60°C.
4. Add 25 mg ampicillin and swirl or stir on a hot plate to mix.
5. Pour approximately 10 mL of molten LB agar into each plate.
6. Allow plates to solidify for approximately 20 minutes.

Can Ampicillin (Cat. No. J60977), be used for administration to or consumption by humans or animals?

Ampicillin, (Cat. No. J60977), is an RUO (Research Use Only) chemical. It is not intended for animal or human diagnostic or therapeutic use.