MitoTracker™ Deep Red FM Dye, for flow cytometry

Q: Is there any difference between the newer MitoTracker dyes for flow cytometry products and the original MitoTracker dye products?

The newer MitoTracker dyes for flow cytometry and the original MitoTracker dyes differ only in the packaging. The original MitoTracker dye products are shipped as 20 vials with 50 µg per vial. The newer MitoTracker dyes for flow cytometry products are shipped as 5 vials with a proprietary amount of dye per vial. The amount of dye per vial is optimized for flow cytometry experiments. Each vial provides sufficient reagent for 20 flow cytometry experiments. Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center .

MitoTracker™ Deep Red FM Dye, for flow cytometry - FAQs

View additional product information for MitoTracker™ Deep Red FM Dye, for flow cytometry - FAQs (M46753)

3 product FAQs found

Our facility does not allow flow cytometry of live cells. Can I fix the cells after staining with MitoTracker Deep Red FM dye?

Yes, this is possible because MitoTracker Deep Red FM dye is retained upon fixation. See Table 2 in this manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp07510.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference in using MitoTracker dyes for flow cytometry compared to imaging?

Because of the greater sensitivity of a flow cytometer, you typically use a much lower concentration of reagent than what is typically used for imaging applications. The MitoTracker dyes, for flow cytometry have been optimized for ease of use for most flow cytometry applications.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is there any difference between the newer MitoTracker dyes for flow cytometry products and the original MitoTracker dye products?

The newer MitoTracker dyes “for flow cytometry” and the original MitoTracker dyes differ only in the packaging. The original MitoTracker dye products are shipped as 20 vials with 50 µg per vial. The newer MitoTracker dyes “for flow cytometry” products are shipped as 5 vials with a proprietary amount of dye per vial. The amount of dye per vial is optimized for flow cytometry experiments. Each vial provides sufficient reagent for 20 flow cytometry experiments.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.