Is the Z-DEVD-R110 substrate in the EnzChek Caspase-3 Assay Kit #2 cell permeable?
It is not cell permeable. You either have to use this product with cell lysates or extracted, purified caspase-3. Cell permeable alternatives are CellEvent Caspase 3/7 Green Detection Reagent (Cat. Nos. C10423, C10427, or R37111), Image-iT LIVE Red or Green Caspase-3 and -7 Detection Kits (Cat. Nos. I35102 and I35106) and Vybrant FAM Caspase-3 and -7 Assay Kit (Cat. No. V35118).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.