Comparison of SuperSignal Western Blot Enhancer with other commercially available enhancers

HeLa cell lysate was separated by electrophoresis, transferred to nitrocellulose (Part No. 88013) and Western blotting was performed using the conventional method (A), SuperSignal Western Blot Enhancer (B), EMD Biosciences SignalBoost™ Immunoreaction Enhancer Kit (C) or Millipore Chemilucent™ Plus Western Blot Enhancing Kit (D) according to manufacturers' directions. Blots were probed with rabbit anti-MAP Kinase (Millipore Part No. 06-182) at 1µg/mL followed by horseradish peroxidase-conjugated goat anti-rabbit IgG (Part No. 31460) at 0.1µg/mL. Detection was performed with Pierce ECL Substrate (Part No. 32209) and exposed to film for 10 minutes.

HeLa cell lysate was separated by electrophoresis, transferred to nitrocellulose (Part No. 88013) and Western blotting was performed using the conventional method (A), SuperSignal Western Blot Enhancer (B), EMD Biosciences SignalBoost™ Immunoreaction Enhancer Kit (C) or Millipore Chemilucent™ Plus Western Blot Enhancing Kit (D) according to manufacturers' directions. Blots were probed with rabbit anti-MAP Kinase (Millipore Part No. 06-182) at 1µg/mL followed by horseradish peroxidase-conjugated goat anti-rabbit IgG (Part No. 31460) at 0.1µg/mL. Detection was performed with Pierce ECL Substrate (Part No. 32209) and exposed to film for 10 minutes.

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