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Qdot 525 Probe
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| 5 | 350 or 633 | 695/40 | 300 | 702 | (in buffer) 5 | microscopy, flow cytometry |
Qdot 525 probe is an excellent green-fluorescent label with outstanding brightness and photostability for imaging and flow cytometry applications. With an emission maximum of ~525 nm, Qdot 525 probe can be excited using a 488 nm laser line or standard FITC filter. Optimum imaging results with brighter signals are achieved with a Qdot 525 filter or light cube.
Organic fluorophores and Qdot probes can be readily combined in multiplexed flow cytometry or imaging studies. Longer-wavelength Qdot probes are also ideal for multiplexed western blots.
Thermo Fisher Scientific offers Qdot 705 probe conjugated to a variety of antibodies, peptides, and proteins, optimized for cellular labeling and detection. In addition, antibody labeling kits are available to allow you to generate your own antibody conjugates or probes.
Qdot Label Conjugates for Cell and Tissue Staining Qdot 525 secondary antibodies
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Three-color staining of HeLa cells using fluorescent Qdot nanocrystal conjugates. The intracellular structures in fixed HeLa cells were visualized using a red-fluorescent F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Qdot 655 (nuclei), a yellow-fluorescent F(ab')2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, Qdot 585 (Golgi), and a green-fluorescent Qdot 525 Streptavidin Conjugate (microtubules).
Qdot Probes
Qdot probes are made from nanocrystals of semiconductor material and are broadly compatible with standard fluorescence techniques, but there are some novel aspects to their use that can be optimized to obtain the best possible results. The exceptional brightness and photostability of Qdot probes make it easier to detect low-abundance targets. With narrow and symmetric emission spectra, Qdot probes are also well-suited for multiplexed fluorescence detection using a combination of Qdot probes and organic dyes.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.