MAP2 Antibody (PA5-17646) in IF

Immunofluorescent analysis of MAP2 in the differentiated neurons from H9 ESC-derived NSCs . 2 weeks after differentiation, cells were fixed, permeabilized and stained with a MAP2 rabbit polyclonal antibody (Product ## PA5-17646) at 1:100 dilution (green) and a HuC/HuD mouse monoclonal antibody (Product ## A21271), at a concentration of 5 ug/ml (red) in blocking buffer for at least 1 hour at room temperature, and then incubated with goat anti-rabbit IgG secondary antibody, Alexa Fluor Plus 488 conjugate (Product ## A32731, green) and a donkey anti-mouse IgG secondary antibody, Alexa Fluor 594 conjugate (Product ## A21203, red) at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product ## 62249).

Immunofluorescent analysis of MAP2 in the differentiated neurons from H9 ESC-derived NSCs . 2 weeks after differentiation, cells were fixed, permeabilized and stained with a MAP2 rabbit polyclonal antibody (Product ## PA5-17646) at 1:100 dilution (green) and a HuC/HuD mouse monoclonal antibody (Product ## A21271), at a concentration of 5 ug/ml (red) in blocking buffer for at least 1 hour at room temperature, and then incubated with goat anti-rabbit IgG secondary antibody, Alexa Fluor Plus 488 conjugate (Product ## A32731, green) and a donkey anti-mouse IgG secondary antibody, Alexa Fluor 594 conjugate (Product ## A21203, red) at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product ## 62249).

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