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VR1 Antibody (PA1-29770) in IF
Immunofluorescent analysis of VR1 using a VR1 polyclonal antibody (Product ## PA1-29770) showing expression patterns of TLR4 and CD14 in trigeminal sensory neurons. White arrows depict examples of neurons expressing both markers for each row of three images, and yellow arrows depict examples of neurons that express one but not both markers. Human trigeminal neurons were evaluated for co-localization of TLR4 (Panels A,D), CD14 (Panels B,J), with a marker for the capsaicinsensitive subclass of nociceptors (TRPV1, Panels B,C for TLR4 and Panels K,L for CD14), or a marker of myelinated sensory neurons (N52, Panels E,F). Rat trigeminal neurons were evaluated for co-localization of TLR4 with TRPV1 (Panels G-I) and CD14 with TRPV1 (Panels M-O). The addition of blocking peptide or the deletion of primary or secondary antisera produced a complete loss of signal in both the human and rat tissues. Scale bar is 100 um for Panels A-F and J-L, and 200 um for Panels G-I and M-O.
