GeneArt® CRISPR Products and Services
|Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) systems are revolutionizing the field of genome editing. Able to achieve highly flexible and specific targeting, CRISPR-Cas systems can be modified and redirected to become powerful tools for genome editing in many species, including mammalian cells. We are continuing to expand our suite of genome editing products to span the entire cell engineering workflow, from cell culture and sample preparation to genome modification, to detection and analysis of known genetic variants. Currently we offer our CRISPR-Cas9 in three formats, an all-in-one expression vector, Cas9 mRNA, and complete service for engineering your cell of choice to produce a custom-designed, stable cell line that meets your requirements.
Learn more about CRISPR-based editing
Available GeneArt® CRISPR-Cas9 genome-editing tools.
CRISPR-Cas9 system greatly simplifies genome editing and has great promise in broad applications such as stem cell engineering, gene therapy, tissue and animal disease models, and engineering disease-resistant transgenic plants. When working with mammalian cells and there is no promoter constraints, we recommend using our GeneArt® CRISPR Nuclease Vector. When working with difficult to transfect cell lines and for multiplexing, we recommend using our GeneArt® CRISPR Nuclease mRNA system.
Figure 1. Available GeneArt® CRISPR-Cas9 genome editing tools. The CRISPR-Cas9 system is composed of a short noncoding guide RNA (gRNA) that has two molecular components, a target-specific CRISPR RNA (crRNA) and an auxiliary trans-activating crRNA (tracrRNA). The gRNA unit guides the Cas9 protein to a specific genomic locus via base pairing between the crRNA sequence and the target sequence. Upon binding to the target sequence, the Cas9 protein induces a double-stranded break at the specific target sequence. Following CRISPR-Cas9–induced DNA cleavage, the break can be repaired by the cellular repair machinery using either nonhomologous end joining (NHEJ) or a homology-directed repair mechanism. With target specificity defined by a very short RNA-coding region, the CRISPR-Cas9 system greatly simplifies genome editing and has great promise in broad applications such as stem cell engineering, gene therapy, tissue and animal disease models, and engineering disease-resistant transgenic plants. We offer multiple solutions for genome editing, including CRISPR-Cas9 all-in-one expression vectors, Cas9 mRNA with options for producing target-specific gRNA, transfection reagents, and analysis tools.
|GeneArt® CRISPR mRNA|
|GeneArt® CRISPR nuclease mRNA||15 μg||A25640||Order now|
|GeneArt® Strings™ U6 DNA||> 200 ng||Contact firstname.lastname@example.org|
|GeneArt® Strings™ T7 DNA||> 200 ng||Contact email@example.com|
|Custom in vitro transcribed gRNA||Contact firstname.lastname@example.org|
|GeneArt® CRISPR Nuclease All-in-one vector|
|GeneArt® CRISPR Nuclease Vector with OFP Reporter Kit||10 reactions||A21174||Order now|
|GeneArt® CRISPR Nuclease Vector with CD4 Enrichment Kit||10 reactions||A21175||Order now|
|GeneArt® CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells)||10 reactions||A21177||Order now|
|GeneArt® CRISPR Nuclease Vector with OFP Reporter Kit (with competent cells)||10 reactions||A21178||Order now|
|Custom CRISPR for every gene; we’ll design your target and clone it for you; ready-to-transfect||100 μg||Contact email@example.com|
|GeneArt® Genomic Cleavage Detection Kit||20 reactions||A24372||Order now|
|Lipofectamine® MessengerMax Reagent||0.3 mL||LMRNA003||Order now|
|Lipofectamine® 2000 Transfection Reagent||1.5 mL||11668019||Order now|
|Lipofectamine® 3000 Transfection Reagent||1.5 mL||L3000015||Order now|
|MEGAshortscript™ T7 Transcription Kit||25 reactions||AM1354||Order now|
|MEGAclear™ Transcription Clean-Up Kit||20 reactions||AM1908||Order now|
Want us to design your target oligonucleotide and clone it for you?
Contact us at CRISPR@lifetech.com. We’ll design and provide you with 100 ng of transfection-quality DNA.
For technical questions related to Life Technologies™ GeneArt® CRISPR products, please contact our technical support team at firstname.lastname@example.org.
If you haven’t heard of CRISPR, watch this recorded webinar to get an overview of CRISPR-Cas–mediated genome editing and learn about the GeneArt® CRISPR Nuclease System.
For Research Use Only. Not for use in diagnostic procedures.