MDM2 Antibody (MA1-113) in IHC

Immunohistochemistry analysis of MDM2 showing staining in the cytoplasm and nucleus of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and heated in a 95C water bath for 20 minutes. Following antigen retrieval, tissues were blocked in 10% goat serum in PBS for 30 minutes at room temperature and quenched with Peroxide Suppressor (Product ## 35000) for 30 minutes. Tissues were then probed with a MDM2 monoclonal antibody (Product ## MA1-113) at a dilution of 40ug/mL in blocking buffer for 1 hour at room temperature. Tissues were washed extensively in PBST and detection was performed using the SuperPicture HRP Polymer Detection Kit (Product ## 87-8963) and DAB substrate (Product ## 34002). Tissues were counterstained with hematoxylin (Product ## TA-125-MH) and dehydrated with ethanol and xylene to prep for mounting.

Immunohistochemistry analysis of MDM2 showing staining in the cytoplasm and nucleus of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and heated in a 95C water bath for 20 minutes. Following antigen retrieval, tissues were blocked in 10% goat serum in PBS for 30 minutes at room temperature and quenched with Peroxide Suppressor (Product ## 35000) for 30 minutes. Tissues were then probed with a MDM2 monoclonal antibody (Product ## MA1-113) at a dilution of 40ug/mL in blocking buffer for 1 hour at room temperature. Tissues were washed extensively in PBST and detection was performed using the SuperPicture HRP Polymer Detection Kit (Product ## 87-8963) and DAB substrate (Product ## 34002). Tissues were counterstained with hematoxylin (Product ## TA-125-MH) and dehydrated with ethanol and xylene to prep for mounting.

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