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Histone H2A-UB Antibody (720148) in ChIP
Enrichment of endogenous Histone H2A-Ub protein using Anti- Histone H2A-Ub Rabbit Polyclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti- Histone H2A-Ub Rabbit Polyclonal Antibody (Product # 720148, 3 µg) on sheared chromatin from 2 million HeLa cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG (1 ug) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the region of inactive SAT2 satellite repeat, used as positive control target, and promoters of the active cFOS, B-Actin region used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
