Acetyl-Histone H2B (Lys 5) Antibody (710812) in WB

Western blot analysis of Histone H2B Lysine 5 acetylation was performed by loading 40 µg of Nuclear protein extracts from BEAS-2B (lane 1) and HeLa (lane 2) in sample buffer and 5µL Spectra Mµlticolor Broad Range Protein Ladder(Product # 26634) onto a 15% polyacrylamide gel . Proteins were transferred to PVDF membrane (Product # 88518) at 65V for 90 min on ice. Membrane was blocked in 5% Milk in PBST for 1 hour at room temperature. Histone H2B Lysine 5 acetylation was detected at approximately 15 kDa using a Histone H2B Lysine 5 acetylation oligoclonal antibody (Product # 710812) at a dilution of 1:1500 in 0.5% Milk in PBST overnight at 4ºC. Followed by goat anti-rabbit alkaline phosphatase secondary antibody at a dilution of 1:20,000 . Chemiluminescent detection was performed using ECF substrate. Data courtesy of Dr. Fondufe-Mittendorf’s lab.

Western blot analysis of Histone H2B Lysine 5 acetylation was performed by loading 40 µg of Nuclear protein extracts from BEAS-2B (lane 1) and HeLa (lane 2) in sample buffer and 5µL Spectra Mµlticolor Broad Range Protein Ladder(Product # 26634) onto a 15% polyacrylamide gel . Proteins were transferred to PVDF membrane (Product # 88518) at 65V for 90 min on ice. Membrane was blocked in 5% Milk in PBST for 1 hour at room temperature. Histone H2B Lysine 5 acetylation was detected at approximately 15 kDa using a Histone H2B Lysine 5 acetylation oligoclonal antibody (Product # 710812) at a dilution of 1:1500 in 0.5% Milk in PBST overnight at 4ºC. Followed by goat anti-rabbit alkaline phosphatase secondary antibody at a dilution of 1:20,000 . Chemiluminescent detection was performed using ECF substrate. Data courtesy of Dr. Fondufe-Mittendorf’s lab.

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