Purification of IgG from human serum

Human Serum was loaded at 80% dynamic binding capacity of POROS MabCapture A Select, MabSelect SuRe LX (GE Healthcare), and Pierce Protein A Plus Agarose. Resins were equilibrated with 10 column volumes and washed with 10 column volumes of Binding Buffer (100 mM phosphate, 150 mM sodium chloride, pH 7.2). IgG was eluted from the resins with 10 column volumes of Elution Buffer (Thermo Scientific Pierce IgG Elution buffer pH 2.0). Experimental conditions: Column area: 0.5 cmD x 5 cmL, Flow rate: 300 cm/hr.

Human Serum was loaded at 80% dynamic binding capacity of POROS MabCapture A Select, MabSelect SuRe LX (GE Healthcare), and Pierce Protein A Plus Agarose. Resins were equilibrated with 10 column volumes and washed with 10 column volumes of Binding Buffer (100 mM phosphate, 150 mM sodium chloride, pH 7.2). IgG was eluted from the resins with 10 column volumes of Elution Buffer (Thermo Scientific Pierce IgG Elution buffer pH 2.0). Experimental conditions: Column area: 0.5 cmD x 5 cmL, Flow rate: 300 cm/hr.

Related Products

Related Images

CD335 (NKp46) Antibody (63335182) in RE Go ›

CD223 (LAG-3) Antibody (56223942) in TM Go ›