Search
Rabbit IgG (H+L) Antibody (A11369) in WB
Goat anti-Rabbit IgG (H+L) Alexa Fluor 790 Secondary Antibody in WB Western blot analysis of total Cadherin and N-Cadherin was performed by loading 2ul SeeBlue® Plus2 Prestained Protein Ladder (Product ## LC5925), 50ug of MDCK cell lysates and 10ug mouse heart lysate per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 1% BSA/TBST for at least 1 hour at room temperature. Total cadherin was detected using a rabbit antibody (Product ## 71-7100) and N-Cadherin was detected using a mouse antibody (Product ##33-3900), both at a concentration of 1ug/ml in blocking buffer overnight at 4°C on a rocking platform. The blot was then incubated with goat anti-rabbit IgG-Alexa Fluor 790 secondary antibody (Product ## A11369) and goat anti-mouse IgG-Alexa Fluor 680 secondary antibody (Product ##A21058) at a dilution of 1:10,000 for at least 1 hour. Fluorescent detection was performed using the Odyssey® CLx imaging system (Li-cor Biosciences). Images generated by Joell Solan in Paul Lampe Lab at Fred Hutchinson Cancer Research Center.
