Photobleaching resistance for fluorescent dyes and proteins with SlowFade® antifade reagents

Photobleaching resistance was quantified on an LSM 710 (Zeiss) confocal microscope. HeLa or U2OS cells were stained and mounted using standard ICC protocols. Five regions within three fields of view were scanned 50x with a 1.58 µs dwell time per pixel. Excitation wavelength and intensity were optimized for the fluorophore being measured.

Photobleaching resistance was quantified on an LSM 710 (Zeiss) confocal microscope. HeLa or U2OS cells were stained and mounted using standard ICC protocols. Five regions within three fields of view were scanned 50x with a 1.58 µs dwell time per pixel. Excitation wavelength and intensity were optimized for the fluorophore being measured.

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