New cryo-PFIB for cellular cryo-electron tomography

Cellular cryo-electron tomography  is a high-resolution technique that enables imaging of a cell’s molecular machinery at close-to-native conditions. With the advent of dedicated cryo-FIB instruments, modern energy filters, fast direct electron detectors, and advanced data acquisition, exciting new opportunities have opened for cryo-electron tomography to shed light on fundamental questions in cell and in situ structural biology. In this webinar, we will present our next-generation Thermo Scientific Arctis Cryo-PFIB , which pioneers the systematic production of lamellae from cellular samples for cryo-electron tomography. The new microscope features a state-of-the-art plasma ion source, an automatic sample loading system (Autoloader), integrated fluorescence microscopy for correlative imaging, and direct connectivity to the cryo-transmission electron microscope. This enables the production of cryo-lamellae with higher throughput and better productivity than ever before.

Cryo-electron tomography sample preparation webinar

Learn more about the Arctis Cryo-Plasma-FIB in this 30 minute on-demand webinar, including:

  • How cryo-electron tomography enables insights into intact cellular environments
  • How the Arctis Cryo-PFIB works
  • How the Arctis Cryo-PFIB is used for sample preparation in cryo-electron tomography


Alex Rigort

Alex Rigort

Product Marketing Manager, Thermo Fisher Scientific

Alex is a product marketing manager for Thermo Fisher Scientific. He developed and used cryo-focused ion beam instrumentation for applications in electron tomography at the Max Planck Institute of Biochemistry in Munich, Germany, and has more than 15 years of experience in cryo-electron microscopy.

Michael Grange

Michael Grange

Group Leader, The Rosalind Franklin Institute, UK

Michael completed his DPhil in structural biology at the University of Oxford, UK, where he applied FIB milling, electron cryo-tomography, and super-resolution microscopy to investigate the trafficking and egress of viral progeny within cells. After his doctoral studies, he moved to the Max Planck Institute for Molecular Physiology, Germany, as an EMBO Long-Term Fellow. During his fellowship, he established cryo-ET as a core method, was involved in establishing high-throughput FIB-milling workflows, and combined both to investigate the structure and architecture of isolated mammalian muscle and human-stem-cell-derived cardiomyocytes. In 2021, he joined the Franklin as a group leader where his research uses in-situ structural techniques to determine the impact of disease-relevant genetic alterations on the structural cell biology of axonal plasticity. He also develops methods for cryo-ET and the correlative imaging of larger tissues for structural studies.

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