Thermo Fisher Scientific live presentations at International Microscopy Congress 2023

Amira Software: Get actionable results from your biological imaging data

Thermo Scientific Amira Software is a powerful, comprehensive, and versatile 2D–5D solution for visualizing, analyzing, and understanding life sciences and biomedical research data from many image modalities. With incredible speed and flexibility, Amira Software supports advanced 2D–5D bioimaging workflows in research areas ranging from structural and cellular biology to tissue imaging, neuroscience, preclinical imaging, and bioengineering. From any 3D image data, including time series and multi-channel, Amira Software delivers a comprehensive range of data visualization, processing, and analysis capabilities. Amira Software allows life sciences and biomedical researchers to gain invaluable insights into their data, at different scales and from any modality.   
   

Glacios 2 Cryo-TEM: A high-resolution structure determination tool with unparalleled automation and ease of use

The improved Thermo Scientific Glacios 2 Cryo-TEM is a 200 kV FEG system built for delivering high throughput and high-resolution structures of proteins. The instrument is equipped with the Thermo Scientific Selectris Imaging Filter and Falcon 4i Direct Electron Detector for the highest image quality, fringe-free imaging, and aberration-free image shift for high throughput data collection. The Glacios 2 Cryo-TEM was designed with ease of use for new users and optimized for supporting data collection on multiple grids. The system can also be used for cryo-ET, allowing cellular structure biology at high resolutions.    
   

Advancing high resolution structure determination of challenging proteins using the new Falcon C Detector with the Tundra Cryo-TEM

Operating at 100 kV, the Thermo Scientific Tundra Cryo-Transmission Electron Microscope (Cryo-TEM) delivers simplified data collection with Thermo Scientific Smart EPU Software and rapid sample loading and unloading. Here, we present the first high-resolution structures of different proteins, including highly symmetric to sub-100 kDa asymmetric proteins obtained from this microscope.   
   

Latest updates for Smart EPU and Tomography software: Increasing automation and ease of use for Cryo-EM data acquisition

Acquiring high-quality data for single particle analysis or cryo-ET still largely depends on the microscope operator’s ability to judge microscope output and optimize the acquisition process. The dependence on this empirical expertise emphasizes the need for more robust automation and real-time feedback throughout the entire data acquisition process. In this presentation, we will present the latest developments on our solutions for SPA and cryo-ET data acquisition: Thermo Scientific Smart EPU and Tomo/Tomo Live Software. These advancements are aimed to simplify and automate processing and data curation while also opening possibilities for AI automation in both workflows.
      

Unlocking the secrets of the cell: A high-throughput collaborative in situ cryo-ET project

Cryo-electron tomography (cryo-ET) has become the preferred method for investigating the structures of biomolecules in their native context. However, the current method of producing thin sections through cryo-focused ion beam milling has limitations that can compromise data quality and volume. A next-generation cryo-plasma FIB improves both lamellae quality and throughput, combining with recent advances in data collection and processing to help you collect large in situ datasets in a reasonable time frame.    
   

Pushing the boundaries in volume EM and cryo-tomography preparation with Hydra Bio Plasma-FIB

The new Thermo Scientific Hydra Bio Cryo-PFIB integrates proven and established cryo technology for TEM lamella preparation with volumetric imaging applications on a highly versatile plasma FIB/SEM. Cryo Auto Slice & View enables the acquisition of high-resolution 3D data through automated serial milling and imaging, enabling striking new insights into a range of frozen-hydrated specimens (prepared by high-pressure freezing or plunge freezing). The choice of four different ion species offers advantages for imaging challenging (for example charging) cryo- and resin-embedded samples. The Hydra Bio also features Spin Mill, an exclusive technique for large-area planar milling that yields similar sized imaging areas as microtome slicing. Hydra Bio brings together field-proven and new techniques to enable both cryo and room temperature work with maximum flexibility. The versatility of the system is further emphasized by the ability to fully support cryo-tomography lamella preparation, cryo-lift-out applications and correlative light and electron microscopy (CLEM) workflows using the iFLM integrated light microscope.    

Automated, high-quality, Ga-free TEM sample preparation for all users in under an hour

Preparation of thin, Ga-free S/TEM samples using focused ion beam (FIB) milling is still considered a challenging task that requires a high level of expertise. The Thermo Scientific Helios 5 Hydra FIB-SEM, in combination with Thermo Scientific AutoTEM 5 Software, significantly simplifies the workflow and enables automated processing of high-quality, Ga-free lamellae for a broad range of materials. Join us to see how the Helios 5 Hydra FIB-SEM’s unique capability to tailor the ion species to specific materials, along with its low-energy polishing (down to 500 eV), makes it best in class for site-specific TEM sample prep.
   

Avizo Software: Get actionable results from your materials imaging data

Analyzing and visualizing imaging data can help you better understand your material's structure, properties, and performances. No matter what scale and data modality you use, Thermo Scientific Avizo Software provides optimized workflows for advanced materials characterization and quality control from a single environment. Avizo Software is a suite of universal, reliable, fully automatable, and customizable digital analytical labs that help materials scientists and engineers innovate faster, produce more reliable processes, and develop better performing materials while reducing cost and time to discovery.
   

ChemiSEM Technology: Live chemical imaging for the fastest time to information

Thermo Scientific ChemiSEM Technology centralizes EDS into the heart of the SEM workflow, enabling real-time navigation by chemistry. Join us for an overview of the technology used within the ChemiSEM Scanning Electron Microscope and how it is used to solve complex problems across materials science. 
   

Easy correlation of SEM and XPS data with the CISA Workflow

Understanding both the chemistry and structure of surfaces can be key to understanding how they behave in an application. Join us to see how data from XPS instruments, such as the Thermo Scientific Nexsa G2 Surface Analysis System, can be combined with imaging from SEMs by using Thermo Scientific Maps Software. We'll show just how easy it is to correlate the data from the two instruments using our dedicated Thermo Scientific CISA Workflow. 

The Thermo Scientific IGST (Inert Gas Sample Transfer) Workflow: Observe air-sensitive materials in their native state

Most materials used in clean energy applications are sensitive to air and water, especially battery materials. The Thermo Scientific IGST (Inert Gas Sample Transfer) Workflow protects your samples to allow you to focus on your research rather than look for sample contamination due to improper sample handling. In this short talk, we will introduce the IGST Workflow and discuss the Thermo Scientific CleanConnect System as well as its features and applications.     
    

Fast millimeter scale subsurface and 3D characterization with nanometer resolution

Join us to learn how DualBeam system with fully integrated fs-Laser provides mm-scale subsurface and 3D analysis at nm resolution and enables new workflows such as fast characterization of air sensitive samples and multi-scale correlative microscopy with deep subsurface sample extraction. It produces very high-quality cut face suitable for SEM imaging, or even surface sensitive EBSD mapping directly after the laser processing. This instrument also offers all the capabilities of proven Helios 5 platform, including highest quality Ga-free TEM/APT sample preparation and extreme high-resolution imaging.  
   

Exploring the scope & scale of automated particle and feature analysis in the SEM

Nearly every area of research or industrial production has some kind of “feature problem.” This can come in the form of particles, morphological or compositional defects, or other microstructural features that cause issues in a sample or process. In short, there is a wide spectrum of analysis styles required to identify, classify, and ultimately report on dispersed features within a sample. Because of this, users must balance their needs for speed and flexibility in how a system must be run to provide the correct type of data set to quantify the problem. In this presentation, we will explore the application space for particle and feature analysis and discuss potential solutions to address this interesting and growing need for academia and industry.

Embark on your characterization journey with Maps Software

Scientists and researchers rely increasingly on nanoscale observations to inform the latest advances in research and analysis. It has, however, become apparent that high-resolution observations lose much of their utility in the absence of the larger macroscopic context. Observations from multiple sources must be linked, providing the necessary multi-scale and multi-modal insight for truly valuable data. Thermo Scientific Maps Software provides powerful imaging workflow automation within an easy-to-use and robust platform. With just a few clicks, you can collect impactful data while preserving the context of your observations. Join this short talk and learn how Maps Software provides an integrated approach to automated data acquisition, annotation, and storage across multiple microscope platforms and modalities.   
 

Strategies for minimizing microscope induced artefacts and perturbations

Minimizing electron beam damage strategies and technologies belong in one of three categories: sample protection, maximizing signal detection and maximizing the signal for a given dose. Thermo Fisher Scientific is engaged in all three pathways to enable imaging and analysis of extremely beam sensitive materials such as polymers, 2D materials, zeolites, MOF’s and COF’s (metal and covalent organic frameworks). In this booth presentation we will discuss recent developments but also mention ongoing research and development projects all geared towards electron beam damage mitigation, they consist of both hardware and software solutions. Field-free imaging for magnetic materials will also be covered.  

Recent Advances in Scripting, Automation, Artificial Intelligence, Machine Learning for advanced TEM applications

In the past few years, machine learning and deep learning have matured significantly in performance and speed, becoming integratable into closed-loop automation sequences. Thermo Fisher Scientific's AutoScript, a cross-platform Python-based API, allows to automate and optimize electron microscopy workflows. By employing instance segmentation, we can separate features in an image, count them, and determine properties like size and shape. Incorporating this into automated workflow enables the targeted experimental setting saving the microscopist’s efforts and precious time. In the case of HR TEM imaging, the positions and diameters of atoms can be detected within the automated workflow. The integration of a neural network enables rapid prediction of atomic structures, which can be performed at multiple locations. Additionally, the neural network can help searching for experimentally relevant interfaces. This automation sequence as well provides the opportunity to optimize experimental set-up during the process. The booth presentation will discuss advances at Thermo Fisher Scientific in fields of automation, scripting and deep learning TEM based data acquisition applications.  

Spectra Ultra S/TEM: Providing the ultimate answers to the semiconductor industry’s R&D challenges

As semiconductor technology roadmaps evolve, researchers need a broader range of analytical solutions that can handle an ever-widening range of beam-sensitive sample materials and structures. At the same time, repeatability and data integrity are also critically important. This presentation discusses how the Thermo Scientific Spectra Ultra S/TEM provides analytical answers for the most challenging R&D applications.