Tradeshow
Sep 18, 2022 - Sep 22, 2022
Budapest Congress Center, Hungary
International Symposium on Chromatography 2022

Thank you for joining us at this year's ISC in Budapest. Take a look at what we offered during the conference. Download our scientific posters and oral presentations for your review.

Interested in finding out more about our solutions? Please let us know and we’ll put you in touch with one of our experts.

Lunchtime seminar


Register for our lunchtime seminar today. Seats are limited, so sign up now!

Tuesday, 20 September | 13:30 - 14:30 CEST

Semi-preparative LC purification and analytical characterization of chemical scissors for impurity precision profiling and genomic fingerprinting

Dr. Alexander Schwenger, CureVac and Dennis Köhler, Thermo Fisher Scientific

The impressive success of mRNA vaccines in the Covid-19 pandemic has fueled the hope for further applications that may open a new age of medical therapies. As for any kind of pharmaceutical agent strict control strategies from early process development to final product is an essential precondition. Assembled of several thousand nucleotides, the molecular mass of these compounds is in the megadalton range. The large size coupled with the lability against chemical, enzymatic or even mechanical treatment are responsible for the stability of chemically sensitive mRNAs. Failure in the essential regions such as the Cap structure and poly A tail can have critical consequences on the integrity and/or translation. To handle the challenges of purification and analytical characterizations of these complex molecules, modern fingerprinting techniques utilizing chemical scissors play a leading role by cleaving regions of interest with high precision into qualifiable and quantifiable fragments [1]. The mapping of subsequent digest fingerprints as well as the analysis and purification of synthetic reagents require highly precise analytical and semi-preparative chromatographic methods and instrumentation to facilitate the precise purification and characterization.

1. CureVac AG, patent #WO2020/127959 A1, 25 June 2020

Lunchtime seminar


Register for our lunchtime seminar today. Seats are limited, so sign up now!

Tuesday, 20 September | 13:30 - 14:30 CEST

Semi-preparative LC purification and analytical characterization of chemical scissors for impurity precision profiling and genomic fingerprinting

Dr. Alexander Schwenger, CureVac and Dennis Köhler, Thermo Fisher Scientific

The impressive success of mRNA vaccines in the Covid-19 pandemic has fueled the hope for further applications that may open a new age of medical therapies. As for any kind of pharmaceutical agent strict control strategies from early process development to final product is an essential precondition. Assembled of several thousand nucleotides, the molecular mass of these compounds is in the megadalton range. The large size coupled with the lability against chemical, enzymatic or even mechanical treatment are responsible for the stability of chemically sensitive mRNAs. Failure in the essential regions such as the Cap structure and poly A tail can have critical consequences on the integrity and/or translation. To handle the challenges of purification and analytical characterizations of these complex molecules, modern fingerprinting techniques utilizing chemical scissors play a leading role by cleaving regions of interest with high precision into qualifiable and quantifiable fragments [1]. The mapping of subsequent digest fingerprints as well as the analysis and purification of synthetic reagents require highly precise analytical and semi-preparative chromatographic methods and instrumentation to facilitate the precise purification and characterization.

1. CureVac AG, patent #WO2020/127959 A1, 25 June 2020

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