Tradeshow
Sep 18, 2022 - Sep 22, 2022
Budapest Congress Center, Hungary
International Symposium on Chromatography 2022

We’re excited to be gathering in Budapest after a few years without the ISC conference. Come join us at our booth during exhibit hours to share ideas and see our products and software.

Lunchtime seminar


Register for our lunchtime seminar today. Seats are limited, so sign up now!

Tuesday, 20 September | 13:30 - 14:30 CEST

Semi-preparative LC purification and analytical characterization of chemical scissors for impurity precision profiling and genomic fingerprinting

Dr. Alexander Schwenger, CureVac and Dennis Köhler, Thermo Fisher Scientific

The impressive success of mRNA vaccines in the Covid-19 pandemic has fueled the hope for further applications that may open a new age of medical therapies. As for any kind of pharmaceutical agent strict control strategies from early process development to final product is an essential precondition. Assembled of several thousand nucleotides, the molecular mass of these compounds is in the megadalton range. The large size coupled with the lability against chemical, enzymatic or even mechanical treatment are responsible for the stability of chemically sensitive mRNAs. Failure in the essential regions such as the Cap structure and poly A tail can have critical consequences on the integrity and/or translation. To handle the challenges of purification and analytical characterizations of these complex molecules, modern fingerprinting techniques utilizing chemical scissors play a leading role by cleaving regions of interest with high precision into qualifiable and quantifiable fragments [1]. The mapping of subsequent digest fingerprints as well as the analysis and purification of synthetic reagents require highly precise analytical and semi-preparative chromatographic methods and instrumentation to facilitate the precise purification and characterization.

1. CureVac AG, patent #WO2020/127959 A1, 25 June 2020

Posters


PosterPresenter
The Dual LC concept for productivity increase in various applications and industriesSylvia Grosse
Automated UHPLC method development for mebendazole and related impurities, from method scouting to robustness testingSylvia Grosse
High-throughput analysis of oligonucleotides using a single quadrupole mass spectrometer for quality controlDennis Koehler
HPLC determination of biogenic amines in beer by AQC derivatizationTibor Muellner
A 1D/2D-UHPLC-MS system and its use in the field of drug metabolism and pharmacokineticsSANOFI,
Chris Tuczemski
Ion chromatography mass spectrometry methods for cationic polar pesticides analysisAlexandra Zatirakha
Simultaneous reversed-phase and anion-exchange method scouting for mRNA impurity determinationSabrina Patzelt
Ensuring precise isolation of macromolecules by hyphenating UHPLC separation power with exact fractionation for analytical and semi-preparative LC purificationSabrina Patzelt

Oral presentations


Oral presentationPresenter
The role of universal detection in modern liquid chromatographyTibor Muellner
The incentive of dual injection capabilities for method development and quantitation in heart-cut 2D-LCChris Tuczemskyi
Advances in the design on anion exchange stationary phases for ion chromatography with various bonding chemistriesAlexandra Zatirakha and Christopher Pohl
High aspect ratio pillar array columns for deep proteome profiling at moderate LC pump pressures adapted to be more LC orientedJeff Op de Beeck
Oligonucleotide sequencing by LC-MS/MS: A novel approach to characterization and quality control of mRNA-bases vaccines and biotherapeuticsAlexander Schwahn

Lunchtime seminar


Register for our lunchtime seminar today. Seats are limited, so sign up now!

Tuesday, 20 September | 13:30 - 14:30 CEST

Semi-preparative LC purification and analytical characterization of chemical scissors for impurity precision profiling and genomic fingerprinting

Dr. Alexander Schwenger, CureVac and Dennis Köhler, Thermo Fisher Scientific

The impressive success of mRNA vaccines in the Covid-19 pandemic has fueled the hope for further applications that may open a new age of medical therapies. As for any kind of pharmaceutical agent strict control strategies from early process development to final product is an essential precondition. Assembled of several thousand nucleotides, the molecular mass of these compounds is in the megadalton range. The large size coupled with the lability against chemical, enzymatic or even mechanical treatment are responsible for the stability of chemically sensitive mRNAs. Failure in the essential regions such as the Cap structure and poly A tail can have critical consequences on the integrity and/or translation. To handle the challenges of purification and analytical characterizations of these complex molecules, modern fingerprinting techniques utilizing chemical scissors play a leading role by cleaving regions of interest with high precision into qualifiable and quantifiable fragments [1]. The mapping of subsequent digest fingerprints as well as the analysis and purification of synthetic reagents require highly precise analytical and semi-preparative chromatographic methods and instrumentation to facilitate the precise purification and characterization.

1. CureVac AG, patent #WO2020/127959 A1, 25 June 2020

Posters


PosterPresenter
The Dual LC concept for productivity increase in various applications and industriesSylvia Grosse
Automated UHPLC method development for mebendazole and related impurities, from method scouting to robustness testingSylvia Grosse
High-throughput analysis of oligonucleotides using a single quadrupole mass spectrometer for quality controlDennis Koehler
HPLC determination of biogenic amines in beer by AQC derivatizationTibor Muellner
A 1D/2D-UHPLC-MS system and its use in the field of drug metabolism and pharmacokineticsSANOFI,
Chris Tuczemski
Ion chromatography mass spectrometry methods for cationic polar pesticides analysisAlexandra Zatirakha
Simultaneous reversed-phase and anion-exchange method scouting for mRNA impurity determinationSabrina Patzelt
Ensuring precise isolation of macromolecules by hyphenating UHPLC separation power with exact fractionation for analytical and semi-preparative LC purificationSabrina Patzelt

Oral presentations


Oral presentationPresenter
The role of universal detection in modern liquid chromatographyTibor Muellner
The incentive of dual injection capabilities for method development and quantitation in heart-cut 2D-LCChris Tuczemskyi
Advances in the design on anion exchange stationary phases for ion chromatography with various bonding chemistriesAlexandra Zatirakha and Christopher Pohl
High aspect ratio pillar array columns for deep proteome profiling at moderate LC pump pressures adapted to be more LC orientedJeff Op de Beeck
Oligonucleotide sequencing by LC-MS/MS: A novel approach to characterization and quality control of mRNA-bases vaccines and biotherapeuticsAlexander Schwahn

Register to attend our lunch seminar