Oct 13, 2020
- Oct 29, 2020
Sign up to the upcoming Omics virtual events that will cover a series of presentations from both Thermo Fisher Scientific and industry experts. Talks will cover areas of research that drive the development of vaccines and diagnostics, cancer pathway proteomics, proteogenomics, and immunopeptidomics, the need for standards that will deliver the promise of proteomics for human health and much more!
Upcoming Omics virtual events:
We will be hosting a free virtual event that focuses on harnessing the power of omics to advance scientific research. Each day will have a dedicated theme which will be complemented by a host of presentations from industry experts, followed by roundtable discussions.
Themes per day:
- October 13 | Tackling Virus Research: Why we need Proteomics to Complement Genomics
- October 14 | Proteomics and Cancer: A new perspective
- October 15 | Go beyond: Unleashing the power of proteomics
Alongside the presentation sessions across the three days, there will be virtual booths available to showcase our latest products and allow you the opportunity to have one-to-one conversations with our Thermo Fisher Scientific experts!
As part of our gold sponsorship at this year’s HUPO Connect, we’ll be hosting a virtual seminar session that will feature two presentations.
Accelerating COVID proteomics research using Tandem Mass Tags
Date: October 19
Time: 15:15 - 16:00 UTC (Coordinated Universal Time)
Presenter: Ryan Bomgarden, Ph.D. | Senior Manager, Research & Development | Thermo Fisher Scientific
COVID-19, a disease caused by a novel coronavirus SARS-CoV-2, is responsible for a global pandemic that has affected tens of millions of people worldwide. Upon infection with the virus, patients exhibit a wide variety of symptoms ranging from asymptomatic to severe organ damage and systemic inflammation which can result in death.
Identifying the underlying differences between patients and their response to infection is paramount to determining effective treatments and predicting disease outcomes. Proteomic profiling using Tandem Mass Tags (TMT) is one technology that enables precise measurement of protein abundances from different patient samples and/or treatments.
This seminar will highlight recent advances in TMT reagent workflows and their use in recent applications to measure SARS-CoV-2 viral particle expression over time, changes in host cell signaling upon infection, differences in immune responses of mild COVID-19 versus severe cases, and identify potential drug targets and off-targets.
Presenter: Lars Plate | Assistant Professor of Chemistry and Biological Sciences | Vanderbilt University, Nashville, TN
Human coronaviruses (hCoV) are an increasing global health threat, as evident by the 2002 SARS epidemic caused by SARS-CoV-1, the 2012 MERS outbreak, and the ongoing COVID-19 pandemic caused by SARS-CoV-2. Despite high protein sequence similarity between SARS-CoV-1 and -2, each strain displays distinctive virulence. A better understanding of the basic molecular mechanisms mediating changes in pathogenicity of different hCoV strains is needed to develop antiviral therapeutics.
Here, we profile the virus-host protein-protein interactions of several hCoV non-structural proteins (nsps) that are critical for virus replication. We use tandem mass tag (TMT)-multiplexed quantitative proteomics to sensitively compare and contrast the interactome of nsp2 and nsp4 from three betacoronavirus strains: SARS-CoV-1, SARS-CoV-2, as well as OC43 – a less pathogenic endemic strain associated with the common cold. This approach enabled us to identify conserved host cell binding partners that may be critical for infection. For example, we identified common nsp2 and nsp4 interactors involved in endoplasmic reticulum (ER) calcium signaling and mitochondrial biogenesis, suggesting a new functional role for these proteins in modulating host processes at ER-mitochondria contact sites. At the same time, we identified interactors unique to each strain, such as an E3 ubiquitin ligase complex for SARS-CoV-1 nsp4. Using the enhanced multiplexing capacity of TMTpro, we have now extended our comparative interactomics workflow to other hCoV nsps and additional virus strains.
Our results can reveal unknown roles these hCoV proteins play in the infection cycle, as well as host factors that may mediate the divergent pathogenicity between endemic and epidemic strains. Importantly, the identified common host-dependencies may present new targets for exploration by host-directed antiviral therapeutics.
Omics Winter Webinars | November 10 - 18, 2020
Select from 10 webinars highlighting recent advancements in products and workflows methodologies to enable cutting-edge research in proteomics and metabolomics. Click on the link below for dates, times, and full information.
- Thermo Scientific AcquireX data acquisition workflow
- Structural Elucidation
- Targeted metabolomics
- Chromatographic solutions
- Thermo Scientific Compound Discoverer software
- Native mass spectrometry
- Targeted proteomics
- Thermo Scientific Proteome Discoverer software