Gene Expression Analysis Using TaqMan® Assays
mRNA & Long Non-Coding RNA
TaqMan® Gene Expression Assays consist of a pair of unlabeled PCR primers and a TaqMan® probe with a FAM™ or VIC® dye label on the 5’ end and minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3’ end. RNA from samples of interest is reverse transcribed into cDNA, and the synthesized cDNA serves as the template for real-time PCR.
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