Need to know all the different ways to use KnockOut™ products?

Find protocols, publications, scientific posters and events by clicking the tabs below.


View a selection of peer-reviewed publications utilizing KnockOut™ products:

Publication Number of Citations KnockOut™ Serum Replacement KnockOut™ DMEM KnockOut™ DMEM/F-12
Takahashi K. et al. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007 Nov 30; 131 (5):861-72 3808  
Hamanaka S. et al. (2011) Generation of germline-competent rat induced pluripotent stem cells. PLoS One 2011 6(7):e22008 1758   
Park IH. et al. (2008) Reprogramming of human somatic cells to pluripotency with defined factors. Nature 2008 Jan 10; 451 (7175):141-6 1269  
Xu C. et al. (2001) Feeder-free growth of undifferentiated human embryonic stem cells. Nature 2001; 19, 971-974 1223   
Amit M. et al. (2000) Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture    Developmental Biology 2000; 227, 271–278 1039   
Sato N. et al. (2004) Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor. Nat Med 2004 Jan 01; 10 (1):55-63 1036   
Kim JH. et al. (2003) Generating CNS neurons from embryonic, fetal, and adult stem cells. Methods Enzymol 2003; 365 303-27 998   
Park IH. et al. (2008) Disease-specific induced pluripotent stem cells. Cell 2008 Sep 05; 134 (5):877-86 667  
Kehat I. et al. (2004) Electromechanical integration of cardiomyocytes derived from human embryonic stem cells. Nat Biotechnol 2004 Oct 01; 22 (10):1282-9 572   
Amit M. et al. (2004) Feeder layer- and serum-free culture of human embryonic stem cells. Biol Reprod 2004 Mar 01; 70(3):837-45528 
Huangfu D. et al. (2008) Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2. Nat Biotechnol 2008 Nov 01; 26(11):1269-75509 
Tesar P. et al. (2007) New cell lines from mouse epiblast share defining features with human embryonic stem cells. Nature 2007 Jul 12; 448 (7150) 196-9477 
Li L. et al. (2010) Human dermal stem cells differentiate into functional epidermal melanocytes. J Cell Sci 2010 Mar 15; 123 Pt 6 853-6037 
Koh K. et al. (2011) Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cells. Cell Stem Cell 2011 Feb 04; 8 (2):200-1336  
Zhang N. et al. (2010) Characterization of Human Huntington's Disease Cell Model from Induced Pluripotent Stem Cells. PLoS Curr 2010 Oct 28; 2 RRN119315 

Scientific Posters

View a selection of posters describing the usage of KnockOut™ products for different applications:

Poster Product usage
ISSCR 2012 “Xeno-free Culture Systems for Pluripotent Stem Cells”This poster shows how KnockOut™ DMEM CTS™, KnockOut™ SR XenoFree CTS™, and KnockOut™ SR GF Cocktail CTS™ were used to generate iPSCs using the CytoTune®-iPS Sendai Reprogramming Kit.
ISCT 2011 “Xeno-free Culture Systems for Stem Cells”This poster shows how KnockOut™ DMEM, KnockOut™ SR XenoFree CTS™, and KnockOut™ SR GF Cocktail CTS™ fit into the xenofree workflow for culturing H9 hESCs.
ISSCR 2011 “Generation of Zero-footprint iPSCs in Xeno-Free or Feeder-Free with CytoTune™ -iPS Sendai Reprogramming Kit”This poster shows how the CytoTune™ -iPS Sendai Reprogramming Kit can be used to generate xenofree iPSCs when combined with  KnockOut™ DMEM, KnockOut™ SR XenoFree CTS™, and KnockOut™ SR GF Cocktail CTS™.
ISSCR 2011 “Synthetic surfaces for the culture of human embryonic and adult stem cells”This poster demonstrates the use of different matrices for growing hESCs and iPSCs, such as Geltrex® hESC Qualified, or CELLstart™ CTS™ with different media, including complete KnockOut™ Serum Replacement medium.
ISSCR 2011 “High Quality Episomal Human iPSC derived from Cord Blood with a Zero Footprint: Applications in Serum-Free and Xeno-Free Culture”This poster shows how our Gibco® Episomal hiPSC Line has applications in serum-free culture, using KnockOut™ Serum Replacement.

Trainings & Events

LifeLab™ Pluripotent Stem Cell Training Events
  • February 25-27, 2014 Carlsbad, CA
  • April 8-10, 2014 Glasgow, UK
  • May 13-15, 2014 Madison, WI
  • August 12-14, 2014 Carlsbad, CA
  • October 7-9, 2014 Glasgow, UK
  • November 4-6, 2014 Madison, WI

View all workshops >


For research use only. Not intended for any animal or human therapeutic or diagnostic use.