Photostability is a key characteristic of Alexa Fluor dye conjugates, allowing more time for image capture. In addition to their superior brightness and the wide selection of dyes across the spectrum, you'll get photostability you can rely on with Alexa Fluor dyes and dye conjugates.
The photostability of the Alexa Fluor dye conjugates is particularly apparent when compared to traditional fluorophores such as fluorescein (Figures 1 and 2).
Figure 2. Reduced photobleaching, sustained signal. Bovine pulmonary artery endothelial cells were labeled with fluorescein phalloidin (A) or Alexa Fluor 488 phalloidin (B) which label filamentous actin. The cells were placed under constant illumination on the microscope. Images were acquired at one-second intervals for 30 seconds. Under these illumination conditions, fluorescein images are photobleached to about 20% of its initial value, while fluorescence of Alexa Fluor 488 phalloidin stayed at the initial value.
Outperforming so-called “Premium” Fluorophores
We have also shown that Alexa Fluor 488 conjugates outperform many recently introduced “premium” fluorophores (Figure 3). Although several conjugates tested showed good photostability, none outperformed the Alexa Fluor 488 dye. Several competing conjugates have significantly lower signal intensity, even under optimal conditions.
Figure 3. Photobleaching analysis results. Mitochondria in bovine pulmonary artery endothelial cells were detected using an anti-OxPhos Complex V inhibitor protein antibody and secondary antibody conjugate of the dye indicated in the graph. Samples were continuously illuminated and data collected every ~250 milliseconds. The working concentrations are noted in the legend.
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