Recommended filters for use with Qdot® nanocrystals

  Omega Optical Filter Sets Chroma Technology Filter Sets
Color Optimal Filter Sets Useable Filter Sets * Optimal Filter Sets Useable Filter Sets *
800†XF307 Qdot® 800 filter set
Exciter 1: 425DF45 or
Exciter 2: 415WB100
Dichroic: 475DCLP
Emitter: 800WB80
XF308 Qdot® 800 filter set for multiplexing
Exciter 1: 425DF45 or Exciter 2: 415WB100
Dichroic: 475DCLP
Emitter: 840WB80
Qdot® 800 filter set
(30 nm EM; 32020)
(460SPUV/475DCXRU
/D800/30nm)
Qdot® 800 filter set
(50 nm EM; 32021)
(460SPUV/475DCXRU
/D800/50nm)
Cy7 (41009)
Li-Cor for IRDye 800 (41037)
Cy7 (SP106)
705†XF306 Qdot® 705 filter set
Exciter 1: 425DF45 or
Exciter 2: 415WB100
Dichroic: 475DCLP
Emitter: 710AF40
XF140-2
XF70
XF110-2
XF141-2
XF48-2
Qdot® 705 filter set
(20 nm EM; 32014)
(460SPUV/475DCXRU/
D705/20nm)
Qdot® 705 filter set
(40 nm EM; 32015)
(460SPUV/475DCXRU/
D705/40nm)
Cy5 longpass (41024) Cy5 (41008)
Cy5 narrow excitation (41033)
Cy5.5 (41023)
Alexa Fluor® 680 (41042)
Cy5.5 (red-shifted; 41022)
655XF305 Qdot® 655 filter set
Exciter 1: 425DF45 or
Exciter 2: 415WB100
Dichroic: 475DCLP
Emitter: 655WB20
XF102-2
XF40-2
XF42
XF45
Qdot® 655 filter set
(20 nm EM; 32011)
(460SPUV/475DCXRU/
D655/20nm)
Qdot® 655 filter set
(40 nm EM; 32012)
(460SPUV/475DCXRU/
D655/40nm)
Texas Red® (41004)
Propidium iodide (41005)
Fura Red™ (31012)
Chlorophyll (31017)
Allophycocyanin (31006)
605XF304 Qdot® 605 filter set
Exciter 1: 425DF45 or
Exciter 2: 415WB100
Dichroic: 475DCLP
Emitter: 605WB20
XF108-2
XF102-2
XF103-2
Qdot® 605 filter set
(20 nm EM; 32003)
(460SPUV/475DCXRU/
D605/20nm)
Qdot® 605 filter set
(40 nm EM; 32007)
(460SPUV/475DCXRU/
D605/40nm)
Cy3 narrow excitation (41007a)
Texas Red®/Cy3.5 (31004)
TRITC (41002, 41002a, 41002b)
Ethidium bromide (41006)
585XF303 Qdot® 585 filter set
Exciter 1: 425DF45 or
Exciter 2: 415WB100
Dichroic: 475DCLP
Emitter: 585WB20
XF101-2
XF137-2
XF152-2
Qdot® 585 filter set
(20 nm EM; 32004)
(460SPUV/475DCXRU/
D585/20nm)
Qdot® 585 filter set
(40 nm EM; 32008)
(460SPUV/475DCXRU/
D585/40nm)
R-PE (41003)
Rhodamine LP (41032)
FITC/PI (41016)
565XF302 Qdot® 565 filter set
Exciter 1: 425DF45 or
Exciter 2: 415WB100
Dichroic: 475DCLP
Emitter: 565WB20
XF104-2
XF105-2
Qdot® 565 filter set
(20 nm EM; 32005)
(460SPUV/475DCXRU/
D565/20nm)
Qdot® 565 filter set
(40 nm EM; 32009)
(460SPUV/475DCXRU/
D565/40nm)
Eosin (41011)
Cascade Yellow™ (31038)
JP2 (YGFP with EGFP; 31040)
Auramine (31015)
525XF301 Qdot® 525 filter set
Exciter 1: 425DF45 or
Exciter 2: 415WB100
Dichroic: 475DCLP
Emitter: 525WB20
XF100-3
XF100-2
XF115-2
XF89-2
Qdot® 525 filter set
(20 nm EM; 32006)
(460SPUV/475DCXRU/
D525/20nm)
Qdot® 525 filter set
(40 nm EM; 32010)
(460SPUV/475DCXRU/
D525/40nm)
FITC/RSGFP/Bodipy®/
Fluo-3/DiO (41001)

FITC/RSGFP longpass (40012)
BFP to GFP FRET (31032)
BFP to GFP FRET wide excitation (31034)
GFP wide blue excitation (31054)
All colors ‡XF300 Qdot® filter set
Exciter 1: 425DF45 or
Exciter 2: 415WB100 Dichroic: 475DCLP
Emitters: 800WB80, 840WB80, 710AF40, 655WB20, 605WB20, 585WB20, 565WB20, 525WB20
XF129-2 XF130-2 Qdot® Multiple Emission Set
(71014)
(460SPUV, 475DCXRU, D525/20nm, D605/20nm, D565/20nm, D585/20nm)
UV (11000V2)
Blue/Violet (11003V2)
UV/Violet (11011V2)
* Useable filter sets will usually provide substantially lower signal than optimal filter sets; sensitive applications will generally require the optimal filter sets.† 705 and 800 nm Qdot® nanocrystal emission cannot be seen by eye and must be detected with an IR-sensitive detector. ‡ For viewing multiple colors of Qdot® nanocrystals through microscope eyepieces.