Go directly from gDNA extraction to PCR with ChargeSwitch Direct
No beads, no membranes, no centrifugation, no vacuum manifold—not even a transfer between tubes or plates. Get your results faster than ever. With the ChargeSwitch Direct 96 gDNA Kit you’ll save time by going directly from genomic DNA (gDNA) isolation to PCR in one well, in less than 45 minutes.
- Save time—eliminate elution steps and extract and amplify in the same well
- Pure DNA you can count on—extraction of genomic DNA without reagents that can inhibit PCR
- Ideal for small-scale quantity isolations—save precious samples for other applications
ChargeSwitch Direct 96 gDNA to PCR workflow
High-performance gDNA without elution, contamination, and false positives
ChargeSwitch kits come with aqueous buffers that do not contain guanidine or ethanol, which can inhibit downstream applications. Genomic DNA can be isolated from as little as 10 µL of whole blood or 1–2 x 104 cells per well. Purified gDNA is amplified directly from microplate wells, without elution, and is suitable for downstream procedures (such as multiplex PCR, qPCR, STR analysis, WGA (whole genome amplification), and sequencing). In addition, ChargeSwitch Direct 96 gDNA Kits are compatible with most automated robotic platforms, and contain semi-skirted microplates that fit into most thermal- and real-time- cyclers for high-throughput procedures. With the ChargeSwitch Direct 96 gDNA Kit, you get successful, high-purity gDNA results with less sample, contamination, sample error, and false positives (Figure 1).
ChargeSwitch Direct 96 competitive analysis
Using the ChargeSwitch Direct 96 gDNA Kit, you can lyse and purify gDNA from up to 96 samples in <45 minutes. Add PCR components directly to the wells of the same plate. The elimination of transfer steps means a reduced risk of contamination and sample mix-ups. (Figure 2).
For Research Use Only. Not for use in diagnostic procedures.