Immunoprecipitation Myths | Thermo Fisher Scientific

Fact: Almost all Background is Removed with Dynabeads® Magnetic Beads

Simplicity is the key.
It seems to be an established fact that background can’t be avoided. Or is this a myth that now ought to be busted?

Watch this video and learn how background can be absolutely avoided with Dynabeads® magnetic beads.

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Fact: Pre-clearing is not Necessary with Dynabeads® Magnetic Beads

Is it really necessary to pre-clear your sample prior to immunoprecipitation?

Let’s say you have made the assumption that some proteins will always bind nonspecifically to your solid phase. In order to remove these proteins, you need to pre-clear the sample, right?

Watch this video and learn how you can avoid background without any pre-clearing.

Fact: High Capacity is not Always a Good Thing

When we say capacity is crucial, we are typically talking about the antigen-binding capacity. Because of the history of protein isolation and the move from large-scale protein purification to immunoprecipitation, people tend to believe that this capacity actually is crucial—when in fact capacity isn’t always a good thing.

The fact is that you want to balance the capacity, yield, specificity, and cost.
Watch this video to learn why.

Fact: You Can Save Money Using Dynabeads® Magnetic Beads

Some people think that Dynabeads® magnetic beads are expensive, while slurry is typically considered to be cheap. Yet the price per sample is comparable—or even lower—when you include the pre-clearing step.

When you calculate the cost of doing your immunoprecipitation, you typically look at the cost of the solid phase relative to the potential capacity of a fixed volume of that solid phase.

Watch this video to better understand how to calculate the actual cost of your immunoprecipitation experiments.

Choose Your Product:

This YouTube video will help you find the optimal product:

Interactive Selection Guide to Immunoprecipitation

Learn more about immunoprecipitation using Dynabeads®, or follow the below links to the specific products:

Kit with beads & buffers:
Immunoprecipitation Kit Protein A
Immunoprecipitation Kit Protein G

Beads only:
Dynabeads® Protein A
Dynabeads® Protein G

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But remember to get your PROMO CODE first!

Speed:
30 minute IP protocol - in one tube!

Ease-of-use:

No pre-treatment/dilution.
No centrifugation.
No columns

Extremely gentle:
Bound only by affinity interactions, your target protein and protein complexes are preserved intact. The gentle Dynabeads® Protein A / Protein G ensure minimal physical stress, maximum sensitivity and yield, with no loss of target protein.