Immunoprecipitation Myths
Fact: Almost all Background is Removed with Dynabeads Magnetic Beads
Simplicity is the key.
It seems to be an established fact that background can’t be avoided. Or is this a myth that now ought to be busted?
Watch this video and learn how background can be absolutely avoided with Invitrogen Dynabeads magnetic beads.
Fact: Pre-clearing is not Necessary with Dynabeads Magnetic Beads
Is it really necessary to pre-clear your sample prior to immunoprecipitation?
Let’s say you have made the assumption that some proteins will always bind nonspecifically to your solid phase. In order to remove these proteins, you need to pre-clear the sample, right?
Watch this video and learn how you can avoid background without any pre-clearing.
Fact: High Capacity is not Always a Good Thing
When we say capacity is crucial, we are typically talking about the antigen-binding capacity. Because of the history of protein isolation and the move from large-scale protein purification to immunoprecipitation, people tend to believe that this capacity actually is crucial—when in fact capacity isn’t always a good thing.
The fact is that you want to balance the capacity, yield, specificity, and cost.
Watch this video to learn why.
Fact: You Can Save Money Using Dynabeads Magnetic Beads
Some people think that Invitrogen Dynabeads magnetic beads are expensive, while slurry is typically considered to be cheap. Yet the price per sample is comparable—or even lower—when you include the pre-clearing step.
When you calculate the cost of doing your immunoprecipitation, you typically look at the cost of the solid phase relative to the potential capacity of a fixed volume of that solid phase.
Watch this video to better understand how to calculate the actual cost of your immunoprecipitation experiments.
Fact: Almost all Background is Removed with Dynabeads Magnetic Beads
Simplicity is the key.
It seems to be an established fact that background can’t be avoided. Or is this a myth that now ought to be busted?
Watch this video and learn how background can be absolutely avoided with Invitrogen Dynabeads magnetic beads.
Fact: Pre-clearing is not Necessary with Dynabeads Magnetic Beads
Is it really necessary to pre-clear your sample prior to immunoprecipitation?
Let’s say you have made the assumption that some proteins will always bind nonspecifically to your solid phase. In order to remove these proteins, you need to pre-clear the sample, right?
Watch this video and learn how you can avoid background without any pre-clearing.
Fact: High Capacity is not Always a Good Thing
When we say capacity is crucial, we are typically talking about the antigen-binding capacity. Because of the history of protein isolation and the move from large-scale protein purification to immunoprecipitation, people tend to believe that this capacity actually is crucial—when in fact capacity isn’t always a good thing.
The fact is that you want to balance the capacity, yield, specificity, and cost.
Watch this video to learn why.
Fact: You Can Save Money Using Dynabeads Magnetic Beads
Some people think that Invitrogen Dynabeads magnetic beads are expensive, while slurry is typically considered to be cheap. Yet the price per sample is comparable—or even lower—when you include the pre-clearing step.
When you calculate the cost of doing your immunoprecipitation, you typically look at the cost of the solid phase relative to the potential capacity of a fixed volume of that solid phase.
Watch this video to better understand how to calculate the actual cost of your immunoprecipitation experiments.
Choose Your Product
Learn more about immunoprecipitation using Dynabeads, or follow the below links to the specific products
Kit with beads & buffers:
Immunoprecipitation Kit Protein A
Immunoprecipitation Kit Protein G
Beads only:
Dynabeads Protein A
Dynabeads Protein G
Dynabeads benefits
Speed:
30 minute IP protocol - in one tube!
Ease-of-use:
- No pre-treatment/dilution.
- No centrifugation.
- No columns
Extremely gentle:
Bound only by affinity interactions, your target protein and protein complexes are preserved intact. The gentle Dynabeads Protein A / Protein G ensure minimal physical stress, maximum sensitivity and yield, with no loss of target protein.
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Sepharose® is a trademark of GE Healthcare companies.
Immunoprecipitation on BenchPaper - An Exciting Shift!
For Research Use Only. Not for use in diagnostic procedures.
