Isolate any biomolecule–just add your biotinylated ligand
Invitrogen Dynabeads Streptavidin products are the gold standard for capturing, isolating, and handling biotinylated molecules. They are the most cited technology in many applications such as library prep for next-generation sequencing (NGS), DNA, RNA isolations, immunoassays.
High reproducibility, low background, high sensitivity, fast kinetics, and easy to automate make them attractive for a wide variety of applications.
Benefits of Dynabeads Streptavidin products
Advantages of using Dynabeads Streptavidin products
- High reproducibility
- Low background binding
- High sensitivity
- Easily automated
- No centrifugation, precipitation, or filtration—all you need is a magnet
- Robust and gentle, with optimal binding kinetics
- Few handling steps, no sample dilution, minimal sample loss
All Dynabeads products are produced with full control of parameters such as bead size, surface area, iron content, and magnetic mobility. The absence of excess physically adsorbed streptavidin helps ensure negligible leakage, and secures a minimal batch-to-batch variation. The uniform characteristics and unique reproducibility within (CV <3%) and between batches helps reduce costs associated with quality control testing (Figure 1). Whether for your research project or for IVD testing activities, you can rely on the consistent performance of Dynabeads products.
Fast and flexible
Dynabeads Streptavidin enables instant and efficient capture of biotinylated molecules via rapid liquid-phase kinetics. This increases speed and sensitivity compared to filters and plate-based approaches (Figure 2). Dynabeads products are truly spherical and have a large surface area per unit volume. This enables a high and constant binding capacity.
Figure 1. Monosized superparamagnetic Dynabeads magnetic beads. Each bead has an even dispersion of magnetic material, encased within a thin polymer shell. This provides a specific and defined surface for binding your ligand. The true uniformity of all beads within each batch (typical CV <3%) provides consistent physical and chemical properties. Unique batch-to-batch reproducibility (typical CV <5%) enables reproducibility and quality of results.
Figure 2. Dynabeads Streptavidin binding kinetics are superior to traditional microtiter plates. The graph shows % binding of tumor necrosis factor (TNF) to immobilized antibody as a function of time. Courtesy of Dr. N-B Liabakk, Norwegian University of Science and Technology, Trondheim, Norway.
Robust and gentle
Dynabeads streptavidin products feature excellent mechanical and chemical stability. No iron leakage. No inhibition of enzyme activity. The monolayer of recombinant streptavidin helps ensure that the vast majority of biotin-binding sites are left sterically available for binding. The technology is also extremely gentle, allowing isolation of proteins as well as large or unstable complexes. The native state of proteins is preserved, and fragile cells remain viable. Flexible volumes may be used, enabling isolation of low-abundance molecules. A high signal-to noise ratio also contributes to increased sensitivity.
Easy handling – manual or automated
Magnetic separation is surprisingly easy. No tedious centrifugation, precipitation, filtration, or columns. Magnetic handling enables easy washing, separation, and concentration of your target. Excellent dispersion abilities and the lack of magnetic remanence make Dynabeads Streptavidin products ideal for manual as well as automated protocols, including microfluidic systems. Depending on your specific application and target molecule, a direct or indirect capture method is applied.
Nucleic acid purification
Library prep for NGS
Protein and peptide isolation
Specific cell isolation/Exosomes
Capture of biotinylated molecules – how it works
When used in combination with a biotinylated probe / ligand, any target molecule can be captured on the streptavidin-coupled Dynabeads, isolated and further manipulated.
Direct or indirect capture?
Depending on your target molecule and the specific application, the direct or indirect capture method can be applied.
For some applications, using a pre-coupled ligand for direct capture allows you to reuse the Dynabeads and thus further reduce sample preparation costs.
An indirect approach can be of benefit when the concentration of your target is low, the specific affinity is weak or the binding kinetics is slow. In the indirect approach, the probe/ligand is allowed to bind to the target in suspension prior to addition of the beads.
A monolayer of streptavidin is covalently coupled to the Dynabeads, helping to ensure negligible leakage that could otherwise disturb your assay.
Product selection guide
Four different types of Dynabeads streptavidin products are available (Table 1). Your choice should be guided by your sample and target properties, buffers and solutions applied, and specific downstream application needs.
Invitrogen Dynabeads M-280 Streptavidin and MyOne Streptavidin T1 are commonly used for protein and nucleic acid applications.
Invitrogen Dynabeads M-270 Streptavidin and MyOne Streptavidin C1 are preferred for nucleic acid protocols involving high chaotropic salt concentrations, for immunoassays involving small biotinylated antigens, and applications incompatible with BSA.
The smaller Dynabeads MyOne beads offer increased binding capacity and slower sedimentation rate, making them ideal for automated applications.
There's also a Dynabeads Streptavidin trial kit with 1 ml of each bead-type, so you can try them out with your own application.
Table 1. Dynabeads streptavidin products.
|Product||Capacity||Characteristics and properties||Ideal for|
|Dynabeads M-280 Streptavidin||Free biotin:
650–900 pmoles/mg beads
5–10 µg/mg beads
|Dynabeads MyOne Streptavidin T1||Free biotin:
> 1,300 pmoles/mg beads
20 µg/mg beads
650–1,350 pmoles/mg beads
5–10 µg/mg beads
|Dynabeads MyOne Streptavidin C1||Free biotin:
> 2,500 pmoles/mg beads
15–20 µg/mg beads
|Dynabeads kilobaseBINDER Kit||70 pmoles of a 4 kb biotinylated DNA fragment and 80 pmoles of a 10 kb fragment per mg beads||
|Dynabeads Biotin Binder||Processes 2 x 109 cells (PBMC) or 200 mL whole blood||
|CELLection Biotin Binder Kit||Processes 2 x 109 cells (PBMC) or 200 mL whole blood||
|Dynabeads FlowComp Flexi||Processes 2 x 109 cells (MNC) or 80 mL whole blood/buffy coat||
For Research Use Only. Not for use in diagnostic procedures.