Dynabeads products have been used around the world in hundreds of thousands of experiments, cited in over 30,000 publications, and on countless lab shelves. Dynabeads Streptavidin is used in the purification of cells, isolation of proteins, nucleic acid capture and NGS workflows. Check out the full list of products and options in our new Dynabeads brochure.
Benefits of Dynabeads Streptavidin products
- Absolute reproducibility
- Low background binding
- High sensitivity
- Easily automated
- No centrifugation, precipitation, or filtration—all you need is a magnet
- Robust and gentle, with optimal binding kinetics
- Few handling steps, no sample dilution, minimal sample loss
Absolute reproducibility
All Dynabeads products are produced with full control of parameters such as bead size, surface area, iron content, and magnetic mobility. The absence of excess physically adsorbed streptavidin helps ensure negligible leakage and secures a minimal batch-to-batch variation. The uniform characteristics and unique reproducibility within (CV <3%) and between batches helps reduce costs associated with quality control testing (Figure 1). Whether for your research project or for IVD testing activities, you can rely on the consistent performance of Dynabeads products.
Figure 1. Monosized superparamagnetic Dynabeads magnetic beads. Each bead has an even dispersion of magnetic material, encased within a thin polymer shell. This provides a specific and defined surface for binding your ligand. The true uniformity of all beads within each batch (typical CV <3%) provides consistent physical and chemical properties. Unique batch-to-batch reproducibility (typical CV <5%) enables reproducibility and quality of results.
Fast and flexible
Dynabeads Streptavidin enables instant and efficient capture of biotinylated molecules via rapid liquid-phase kinetics. This increases speed and sensitivity compared to filters and plate-based approaches (Figure 2). Dynabeads products are truly spherical and have a large surface area per unit volume. This enables a high and constant binding capacity.
Figure 2. Dynabeads Streptavidin binding kinetics are superior to traditional microtiter plates. The graph shows % binding of tumor necrosis factor (TNF) to immobilized antibody as a function of time. Courtesy of Dr. N-B Liabakk, Norwegian University of Science and Technology, Trondheim, Norway.
Robust and gentle
Dynabeads streptavidin products feature excellent mechanical and chemical stability. No iron leakage. No inhibition of enzyme activity. The monolayer of recombinant streptavidin helps ensure that the vast majority of biotin-binding sites are left sterically available for binding. The technology is also extremely gentle, allowing isolation of proteins as well as large or unstable complexes. The native state of proteins is preserved, and fragile cells remain viable. Flexible volumes may be used, enabling isolation of low-abundance molecules. A high signal-to-noise ratio also contributes to increased sensitivity.
Easy handling – manual or automated
Magnetic separation is surprisingly easy. No tedious centrifugation, precipitation, filtration, or columns. Magnetic handling enables easy washing, separation, and concentration of your target. Excellent dispersion abilities and the lack of magnetic remanence make Dynabeads Streptavidin products ideal for manual as well as automated protocols, including microfluidic systems. Depending on your specific application and target molecule, a direct or indirect capture method is applied.
How capture of molecules and cells with Streptavidin beads works
When used in combination with a biotinylated probe / ligand, any target molecule can be captured on the streptavidin-coupled Dynabeads, isolated and further manipulated.
Direct or indirect capture?
Depending on your target molecule and the specific application, the direct or indirect capture method can be applied.
For some applications, using a pre-coupled ligand for direct capture allows you to reuse the Dynabeads and thus further reduce sample preparation costs.
An indirect approach can be of benefit when the concentration of your target is low, the specific affinity is weak or the binding kinetics is slow. In the indirect approach, the probe/ligand is allowed to bind to the target in suspension prior to addition of the beads.
A monolayer of streptavidin is covalently coupled to the Dynabeads, helping to ensure negligible leakage that could otherwise disturb your assay.
Streptavidin beads by application
| Typical applications | Preferred products | |
|---|---|---|
Nucleic Acid capture |
| |
Protein capture |
| |
Cell capture |
|
Use the chart below to identify the right bead for your sample based on size and automation need
With the Streptavidin beads and the ability to work with the KingFisher allow for no centrifugation steps or columns.
The Dynabeads magnetic beads enable higher-throughput applications, for which samples can be processed in 24- or 96-well plates on the KingFisher family of instruments.
To download streptavidin protocols for yourself visit out library at thermofisher.com/kingfisherprotocols and easily customize them using BindIt Software.
Also to see how the kingfisher and dynabeads are used in the app note Mutation detection sensitivity in matched FFPE tissue and liquid biopsy samples.
Benefits of Dynabeads Streptavidin products
- Absolute reproducibility
- Low background binding
- High sensitivity
- Easily automated
- No centrifugation, precipitation, or filtration—all you need is a magnet
- Robust and gentle, with optimal binding kinetics
- Few handling steps, no sample dilution, minimal sample loss
Absolute reproducibility
All Dynabeads products are produced with full control of parameters such as bead size, surface area, iron content, and magnetic mobility. The absence of excess physically adsorbed streptavidin helps ensure negligible leakage and secures a minimal batch-to-batch variation. The uniform characteristics and unique reproducibility within (CV <3%) and between batches helps reduce costs associated with quality control testing (Figure 1). Whether for your research project or for IVD testing activities, you can rely on the consistent performance of Dynabeads products.
Figure 1. Monosized superparamagnetic Dynabeads magnetic beads. Each bead has an even dispersion of magnetic material, encased within a thin polymer shell. This provides a specific and defined surface for binding your ligand. The true uniformity of all beads within each batch (typical CV <3%) provides consistent physical and chemical properties. Unique batch-to-batch reproducibility (typical CV <5%) enables reproducibility and quality of results.
Fast and flexible
Dynabeads Streptavidin enables instant and efficient capture of biotinylated molecules via rapid liquid-phase kinetics. This increases speed and sensitivity compared to filters and plate-based approaches (Figure 2). Dynabeads products are truly spherical and have a large surface area per unit volume. This enables a high and constant binding capacity.
Figure 2. Dynabeads Streptavidin binding kinetics are superior to traditional microtiter plates. The graph shows % binding of tumor necrosis factor (TNF) to immobilized antibody as a function of time. Courtesy of Dr. N-B Liabakk, Norwegian University of Science and Technology, Trondheim, Norway.
Robust and gentle
Dynabeads streptavidin products feature excellent mechanical and chemical stability. No iron leakage. No inhibition of enzyme activity. The monolayer of recombinant streptavidin helps ensure that the vast majority of biotin-binding sites are left sterically available for binding. The technology is also extremely gentle, allowing isolation of proteins as well as large or unstable complexes. The native state of proteins is preserved, and fragile cells remain viable. Flexible volumes may be used, enabling isolation of low-abundance molecules. A high signal-to-noise ratio also contributes to increased sensitivity.
Easy handling – manual or automated
Magnetic separation is surprisingly easy. No tedious centrifugation, precipitation, filtration, or columns. Magnetic handling enables easy washing, separation, and concentration of your target. Excellent dispersion abilities and the lack of magnetic remanence make Dynabeads Streptavidin products ideal for manual as well as automated protocols, including microfluidic systems. Depending on your specific application and target molecule, a direct or indirect capture method is applied.
How capture of molecules and cells with Streptavidin beads works
When used in combination with a biotinylated probe / ligand, any target molecule can be captured on the streptavidin-coupled Dynabeads, isolated and further manipulated.
Direct or indirect capture?
Depending on your target molecule and the specific application, the direct or indirect capture method can be applied.
For some applications, using a pre-coupled ligand for direct capture allows you to reuse the Dynabeads and thus further reduce sample preparation costs.
An indirect approach can be of benefit when the concentration of your target is low, the specific affinity is weak or the binding kinetics is slow. In the indirect approach, the probe/ligand is allowed to bind to the target in suspension prior to addition of the beads.
A monolayer of streptavidin is covalently coupled to the Dynabeads, helping to ensure negligible leakage that could otherwise disturb your assay.
Streptavidin beads by application
| Typical applications | Preferred products | |
|---|---|---|
Nucleic Acid capture |
| |
Protein capture |
| |
Cell capture |
|
Use the chart below to identify the right bead for your sample based on size and automation need
With the Streptavidin beads and the ability to work with the KingFisher allow for no centrifugation steps or columns.
The Dynabeads magnetic beads enable higher-throughput applications, for which samples can be processed in 24- or 96-well plates on the KingFisher family of instruments.
To download streptavidin protocols for yourself visit out library at thermofisher.com/kingfisherprotocols and easily customize them using BindIt Software.
Also to see how the kingfisher and dynabeads are used in the app note Mutation detection sensitivity in matched FFPE tissue and liquid biopsy samples.
Table 1. Dynabeads streptavidin products.
| Product | Capacity | Characteristics and properties | Ideal for |
|---|---|---|---|
| Dynabeads M-280 Streptavidin | Free biotin: 650–900 pmoles/mg beads Biotinylated Ig: 5–10 µg/mg beads |
|
|
| Dynabeads MyOne Streptavidin T1 | Free biotin: > 1,300 pmoles/mg beads Biotinylated Ig: 20 µg/mg beads |
|
|
| Dynabeads M-270 Streptavidin | Free biotin: 650–1,350 pmoles/mg beads Biotinylated Ig: 5–10 µg/mg beads |
|
|
| Dynabeads MyOne Streptavidin C1 | Free biotin: > 2,500 pmoles/mg beads Biotinylated Ig: 15–20 µg/mg beads |
|
|
| Dynabeads kilobaseBINDER Kit | 70 pmoles of a 4 kb biotinylated DNA fragment and 80 pmoles of a 10 kb fragment per mg beads |
|
|
| Dynabeads Biotin Binder | Processes 2 x 109 cells (PBMC) or 200 mL whole blood |
|
|
| CELLection Biotin Binder Kit | Processes 2 x 109 cells (PBMC) or 200 mL whole blood |
|
|
| Dynabeads FlowComp Flexi | Processes 2 x 109 cells (MNC) or 80 mL whole blood/buffy coat |
|
|
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