Lipofectamine 2000 product photo

The trusted, simple, broad-spectrum transfection reagent for most cell lines

Transfection commonly refers to the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. Invitrogen Lipofectamine 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect a variety of payloads into the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine 2000 reagent for plasmid DNA transfection as well as siRNA- and shRNA-based gene knockdown experiments and gene expression studies.

With Lipofectamine 2000 reagent you can expect:


Transfect your cells with confidence

Lipofectamine 2000 reagent timeline

Figure 1. Outline of protocol steps for the use of Lipofectamine 2000 reagent.

Lipofectamine 2000 reagent delivers DNA or siRNA with excellent transfection performance for protein expression, gene silencing, and functional assays. At the foundation of the popularity of this broad-spectrum reagent is the reliably high efficiency and simple protocol for success across a broad range of cell lines (Figure 1). View the protocols below.

  • For gene silencing, high-efficiency transfection provides the high levels of gene knockdown needed to produce convincing results
  • Lipofectamine 2000 transfection reagent is a superior choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA
  • Ideally suited for high-throughput work because transfection conditions can be easily established for automated or robotic systems

A high-performance workhorse transfection reagent

Lipofectamine 2000 transfection reagent works effectively with all common cell lines (Figure 2) as well as with many challenging ones and can be used in media with or without serum. View the protocols in the “resources” section below.

series of GFP expression after transfection using Lipofectamine 2000

Figure 2. High GFP expression following transfection using Lipofectamine 2000 reagent.


Lipofectamine reagent recognition

Publications citing Lipofectamine 2000 reagent steadily rose after the market introduction in 1999, and this product continues to be one of the most-cited transfection reagents on the market (Figure 3).

graph showing publications citing the use of Lipofectamine 2000 Transfection Reagent
Figure 3. Number of publications citing the use of Lipofectamine 2000 Transfection Reagent since 2000.

Ready for next-generation, enhanced transfection efficiency? Upgrade to Lipofectamine 3000 transfection reagent

Lipofectamine 3000 transfection reagent

The benefits of upgrading from Lipofectamine 2000 reagent to our more-efficient Invitrogen Lipofectamine 3000 transfection reagent include:

  • Superior efficiency into the broadest spectrum of difficult-to-transfect cell types
  • Gentle with low toxicity
  • Superior cell spectrum and higher transfection efficiency to enhance your cancer research
  • Highly efficient reprogramming of somatic cells without the need for electroporation
  • Improved genome editing outcomes
  • Good transfection reagent option for stem cell transfection

Explore the possibilities of using Lipofectamine 3000 regent including protocols and resources now

Resources

User guide

Online protocols

Green fact sheet

General resources for transfection