Lipofectamine 2000 Transfection Reagent

The trusted, simple, broad-spectrum transfection reagent for most cell lines

Transfection commonly refers to the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. Invitrogen Lipofectamine 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect a variety of payloads into the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine 2000 reagent for plasmid DNA transfection as well as siRNA- and shRNA-based gene knockdown experiments and gene expression studies.

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Figure 1. Outline of protocol steps for the use of Lipofectamine 2000 reagent.

Lipofectamine 2000 reagent delivers DNA or siRNA with excellent transfection performance for protein expression, gene silencing, and functional assays. At the foundation of the popularity of this broad-spectrum reagent is the reliably high efficiency and simple protocol for success across a broad range of cell lines (Figure 1). View the protocols below.

• For gene silencing, high-efficiency transfection provides the high levels of gene knockdown needed to produce convincing results
• Lipofectamine 2000 transfection reagent is a superior choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA
• Ideally suited for high-throughput work because transfection conditions can be easily established for automated or robotic systems

Lipofectamine 2000 transfection reagent works effectively with all common cell lines (Figure 2) as well as with many challenging ones and can be used in media with or without serum. View the protocols in the “resources” section below.

Figure 2. High GFP expression following transfection using Lipofectamine 2000 reagent.