Lipofectamine LTX Reagent

Lipofectamine LTX Reagent is a plasmid transfection reagent that offers a balance of potency and gentleness for your cells, resulting in high transfection efficiencies and viabilities. This reagent provides an advanced solution for gene expression in common cell lines, particularly Chinese Hampster Ovary (CHO), and challenging cell types such as primary neural progenitor cells, primary fibroblasts and primary epithelial cells.

Lipofectamine LTX Reagent offers:

  • Superior delivery—transfect common cell lines and challenging primary and sensitive cells, with high efficiency
  • Superior performance—combine high transfection efficiency with maximal (>90%) cell viability
  • Optimized protocol—rapid, simple protocol provided for most cell lines

Broad compatibility with cell lines

In addition to being excellent for transfecting primary and difficult-to-transfect cells, Lipofectamine LTX Reagent provides you with the most versatile approach to transfecting established cells lines, including those that are sensitive to other transfection reagents (Figure 1).

table showing  ratio of peak gene expression to cytotoxicity, with higher ratios indicating optimization of transfection efficiency with low cytotoxicity levels

Figure 1. Lipofectamine LTX Reagent combines high transfection efficiency with low cytotoxicity superior to FuGENE® 6 and FuGENE® HD transfection reagents. Activity/toxicity analysis measures the ratio of peak gene expression to cytotoxicity, with higher ratios indicating optimization of transfection efficiency with low cytotoxicity levels. Ten cell lines were transfected with luciferase-expressing plasmids following recommended protocols for the respective reagents. Cells were analyzed for toxicity using the Invitrogen Vybrant™ Cytotoxicity Assay Kit (Cat. no. V23111) and assayed for luciferase expression. The data were normalized to the activity/toxicity ratios for the FuGENE® HD reagent.

Transfect primary cells efficiently

Primary cells have traditionally been difficult to transfect. Lipofectamine LTX Reagent, when combined with PLUS™ Reagent, solves the problem of transfecting primary cells, including primary neural progenitor cells (Figure 2).

GFP-expressing plasmid in the presence of PLUS Reagent

Figure 2. Lipofectamine LTX Reagent with PLUS™ Reagent efficiently transfects primary neural progenitor cells. Lipofectamine LTX Reagent (1.5 μl) was used to transfect murine embryonic primary neural progenitor cells with the indicated quantities of a GFP-expressing plasmid in the presence of PLUS Reagent (2.5 μl). GFP expression was analyzed 24 hr post-transfection. Data courtesy of the Beverly Davidson laboratory, University of Iowa.

Simple, rapid serum-compatible protocol

Lipofectamine LTX Reagent has a new easy protocol with improved performance. No need to remove transfection complexes, or change medium following transfection. You’ll spend less hands-on time, and get better results, faster.

graph displaying Lipofectamine LTX Reagent with PLUS Reagent performance comparison with new and old protocol
Figure 3. Lipofectamine LTX Reagent with PLUS Reagent performance comparison with new and old protocol.

Meet the inventor

R&D transfection insights

Senior Staff Scientist, Xavier de Mollerat du Jeu discusses the transfection research program and key factors for successful transfections, which resulted in enhanced protocols for the Lipofectamine line of transfection reagents.

Plasmid transfection protocol

How to perform Plasmid DNA transfection with Lipofectamine LTX Reagent with PLUS Reagent

Superior plasmid delivery and protein expression.