Lipofectamine® LTX Reagent is a plasmid transfection reagent that offers a balance of potency and gentleness for your cells, resulting in high transfection efficiencies and viabilities. This reagent provides an advanced solution for gene expression in common cell lines, particularly Chinese Hampster Ovary (CHO), and challenging cell types such as primary neural progenitor cells, primary fibroblasts and primary epithelial cells.
Lipofectamine® LTX Reagent offers:
Transfect primary cells efficiently
Primary cells have traditionally been difficult to transfect. Lipofectamine® LTX Reagent, when combined with PLUS™ Reagent, solves the problem of transfecting primary cells, including primary neural progenitor cells (Figure 1).
Figure 1: Lipofectamine® LTX Reagent, with PLUS™ Reagent, efficiently transfects primary neural progenitor cells. Lipofectamine® LTX Reagent (1.5 μl) was used to transfect murine embryonic primary neural progenitor cells with the indicated quantities of a GFP-expressing plasmid in the presence of PLUS™ Reagent (2.5 μl). GFP expression was analyzed 24 hr posttransfection. Data courtesy of the Beverly Davidson laboratory, University of Iowa.
Broad compatibility with cell lines
In addition to being excellent for transfecting primary and difficult-to-transfect cells, Lipofectamine® LTX Reagent provides you with the most versatile approach to transfecting established cells lines, including those that are sensitive to other transfection reagents (Figure 2).
Figure 2: Lipofectamine® LTX Reagent combines high transfection efficiency with low cytotoxicity superior to FuGENE® 6 and FuGENE® HD transfection reagents. Activity/toxicity analysis measures the ratio of peak gene expression to cytotoxicity, with higher ratios indicating optimization of transfection efficiency with low cytotoxicity levels. Ten cell lines were transfected with luciferase-expressing plasmids following recommended protocols for the respective reagents. Cells were analyzed for toxicity using the Vybrant® Cytotoxicity Assay Kit (Cat. no. V23111) and assayed for luciferase expression. The data were normalized to the activity/toxicity ratios for the FuGENE® HD reagent.
Simple, rapid serum-compatible protocol
Lipofectamine® LTX Reagent has a new easy protocol with improved performance. No need to remove transfection complexes, or change medium following transfection. You’ll spend less hands-on time, and get better results, faster.
Figure 3: Lipofectamine® LTX and Plus™ Reagent performance comparison with new and old protocol.
Meet the inventor
R&D Transfection Insights
Xavier de Mollerat du Jeu, Sr. Staff Scientist at Life Technologies discusses the transfection research program and key factors for successful transfections, which resulted in enhanced protocols for the Lipofectamine® line of transfection reagents.