Superior mRNA transfection efficiency
Introducing Invitrogen's Lipofectamine MessengerMAX mRNA Transfection Reagent, the mRNA transfection reagent with up to 5X the efficiency of DNA reagents in neurons and primary cell types.
- Amazing transfection efficiency in neurons and primary cell types
- Faster protein expression with no risk of genomic integration
- Up to 10X higher cleavage efficiency using mRNA CRISPRs
Amazing transfection efficiency in neurons and primary cell types
Lipofectamine MessengerMAX mRNA Transfection Reagent delivers amazing transfection efficiency in neurons and a broad-spectrum of primary cells, enabling improved application outcomes and more biologically relevant research. That's because our novel lipid nanoparticle technology is optimized to deliver the highest amount of mRNA possible without the nuclear entry step that is required with DNA.
Faster protein expression with no risk of genomic integration
Transfection of mRNA with Lipofectamine MessengerMAX typically results in faster protein expression with great homogeneity of expression among the transfected cells. Additionally, delivery of mRNA does not require nuclear entry (step 4), which eliminates the risk of genomic integration and transfection efficiency becomes cell cycle independent.
Figure 4. DNA vs. mRNA transfection.
Up to 10X higher cleavage efficiency using CRISPR mRNAs
Lipofectamine MessengerMAX reagent increases the likelihood of cleavage and recombination with Invitrogen GeneArt CRISPR Nuclease mRNA through highly efficient transfection, maximizing the efficiency of CRISPR-driven genetic modifications and simplifying downstream processes.
Figure 5. Cleavage efficiency of various Invitrogen GeneArt CRISPR formats targeting the HPRT locus in Gibco iPS cells in a 12-well format. Invitrogen Lipofectamine 3000 reagent was used to deliver CRISPR nuclease all-in-one plasmid DNA; Lipofectamine MessengerMAX and two leading mRNA delivery reagents were used to deliver an all-RNA CRISPR format (Cas9 mRNA + IVT gRNA). Cleavage efficiency was determined using the Invitrogen GeneArt Genomic Cleavage Detection Kit 72 hours post-transfection.
Getting started is easy as 1-2-3!
Prepare DNA template
Prepare your DNA template with a T7 promoter. You may choose to clone your gene with an Invitrogen Gateway pcDNA-DEST40 Vector or amplify it with PCR and T7-containing primers.
Generate mRNA
Transcribe your template DNA with the Invitrogen mMESSAGE mMACHINE T7 ULTRA Transcription Kit to generate mRNA for transfection.
Transfect your mRNA with our simple Lipofectamine MessengerMAX transfection protocol. Download the Lipofectamine MessengerMAX protocol
Prepare DNA template
Prepare your DNA template with a T7 promoter. You may choose to clone your gene with an Invitrogen Gateway pcDNA-DEST40 Vector or amplify it with PCR and T7-containing primers.
Generate mRNA
Transcribe your template DNA with the Invitrogen mMESSAGE mMACHINE T7 ULTRA Transcription Kit to generate mRNA for transfection.
Transfect your mRNA with our simple Lipofectamine MessengerMAX transfection protocol. Download the Lipofectamine MessengerMAX protocol
Cells tested with Lipofectamine MessengerMAX reagent
| Cell type | Lipofectamine MessengerMAX transfection efficiency (%) |
|---|---|
| MDA-MB-231 |  |
| A431 |  |
| A549 | |
| bEnd.3 |  |
| BJ fibroblast |
|
| H9 ESCs |
|
| Primary hepatocytes | |
| Hep G2 | |
| HT-29 | |
| iPSCs | |
| Primary keratinocytes | |
| L929 | |
| LNCaP | |
| hNSCs | |
| MCF7 | |
| Neuro-2a | |
| Primary neurons | |
| RAW 264.7 | |
| RBL |  |
| SK-N-SH | |
| SH-SY5Y | |
Transfection efficiency (%): <30% 30–50% 51–79% >80%
Watch the videos and answer frequently asked questions about working with mRNA
Think mRNA is unstable?
Think mRNA is difficult to prep?
Think mRNA is time consuming?
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mRNA Transfection Basics
Ordering information
In order to generate your mRNA transcript, we recommend the following transcription kit:
To help ensure validity of transfection results, we strongly recommend the use of a positive control mRNA, available from various third party vendors, including TriLink BioTechnologies (e.g., EGFP mRNA).
Sustainable packaging
Packaging must protect the product inside—but that doesn’t have to mean sacrificing sustainability. Lipofectamine products are shipped at ambient temperature, diverting 24,000 ft3 of Expanded Polystyrene (EPS) coolers from landfills annually. We reduce the carbon footprint from packaging and shipping these products by 80 tons (CO2 equivalents).
Learn more about sustainable solutions
Download the green fact sheet
Resources
For Research Use Only. Not for use in diagnostic procedures.
