Best for reading more than 2,000 samples

These Quant-iT™ products provide the maximum amount of dye in packaging that is amenable to highly automated quantitation environments. These are the best choice for quantitating more than 2,000 samples at a time.

These Quant-iT™ products are available in two forms:

  • Concentrated Quant-iT™ dyes
  • Quant-iT™ kits with buffer and a standard included

For quantitation of 20–2,000 samples at a time, see our Quant-it™ Kits for Microplates.

dsDNA specificity of PicoGreen® dsDNA Reagent  

Figure 1. dsDNA specificity of PicoGreen® dsDNA Reagent. Fluorescence enhancement of the Quant-iT™ PicoGreen® dsDNA Reagent upon binding dsDNA, ssDNA, and RNA, demonstrating the strong selectivity of this reagent for dsDNA.

Nucleic acid quantitation using Quant-iT™ Reagents
Figure 2. Nucleic acid quantitation using Quant-iT™ Reagents
. (Left) The fluorescence signal from binding of the Quant-iT™ PicoGreen® dsDNA Reagent to dsDNA is linear over at least four orders of magnitude; the inset shows the results obtained using 0–750 pg/ml calf thymus DNA. (Center) The Quant-iT™ RiboGreen® RNA Assay provides linear quantitation from 20 ng/ml to 1 μg/ml RNA using a 400-fold dilution of the dye; the inset shows the results for 1.0 ng/ml to 50 ng/ml RNA obtained using a 4,000-fold dilution of the dye. (Right) Quantitation of a synthetic 24-mer using the Quant-iT™ OliGreen™ ssDNA Reagent is linear over at least four orders of magnitude; the inset shows the results obtained using 0–2.0 ng/ml of oligo.

Qubit® assays for use with the Qubit® 3.0 Fluorometer

For everyday needs when quantitating 1–20 samples, use Qubit® Assays for use with the Qubit® 3.0 Fluorometer. Ideal for cloning, sequencing, transfection, qPCR, microarrays, protein gels, Western blots and protein activity assays. These kits are formulated for use with the Qubit® 3.0 Fluorometer. Also available is the Qubit® 3.0 Quantitation Starter Kit, which includes the Qubit® 3.0 Fluorometer and four Qubit® Assay Kits.