Quantitation using Quant-iT Assay Kits. (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double the amount shown. The inset shows the sensitivity of the assay for DNA. (Center) The Quant-iT RNA Assay Kit has a linear detection range of 5–100 ng and is selective for RNA, even in the presence of an equal mass of DNA. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double what is stated. (Right) The Quant-iT Protein Assay Kit has a detection range of 0.25–5 μg. This graph shows the signal generated from nine different proteins over the stated detection range. The protein-to-protein variability at the 3 μg point for all nine proteins assayed is 12%.
Quant-iT Reagents and Kits for 20-2,000 Samples
Best for reading 20–2,000 samples
The Quant-iT Kits, 1,000 assay size, provide the ultimate in convenience for DNA, RNA or protein quantitation in microplates. These are the best choice for quantitating 20–2,000 samples at a time.
The Quant-iT Kits for use with microplate readers include:
- Concentrated Quant-iT dyes for the highest accuracy and sensitivity
- Eight pre-diluted standards for generating the standard curve
- Ready-to-use 1x buffer
- Standardized protocols for automation-level efficiency, even for labs without robotics
Expertly detect fluorescence with Thermo Scientific plate readers
High-sensitivity fluorescence detection of nucleic acids or protein for 96-1536 samples can be quickly performed on Fluoroskan or Fluoroskan FL Microplate Fluorometer or Varioskan LUX Multimode Microplate Reader using Invitrogen reagents for optimal detection. Take advantage of automatic dynamic range selection to get optimal gain settings for each individual well and automation capabilities for even higher throughput.
Featured application: Sensitive DNA quantification
Read about detection limit (LOD) as low as 0.04ng of DNA in this featured application note highlighting sensitive fluorescence detection of DNA capabilities when you combine Quant-iT technology with a Fluoroskan plate reader.
Qubit assays for use with the Qubit fluorometers
For everyday needs when quantitating 1–20 samples, use Invitrogen Qubit Assays for use with the Qubit fluorometers. Ideal for cloning, sequencing, transfection, qPCR, microarrays, protein gels, Western blots and protein activity assays. See how easy it is to use the latest version of the Qubit - the Qubit 4 fluorometer.
For Research Use Only. Not for use in diagnostic procedures.