Spotlight Articles–Thermo Scientific Molecular Biology

Browse and learn from our spotlight articles containing practical technical tips to help you get the meaningful results you need at the bench. This collection of articles spans PCR, molecular cloning, reverse transcription, and more. Come back often as we are always adding new content to help you advance your molecular biology applications.

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Category	Article
Reverse transcription	Overcoming hurdles in cDNA synthesis 3 ways to boost reverse transcriptase enzyme performance
Reverse transcription	Understanding the Molecular Evolution of a Reverse Transcriptase Directed evolution helped build a better reverse transcriptase
Isothermal amplification	The long and short of isothermal amplification Learn the advantages of LAMP and WGA
PCR	3 questions to ask when choosing a PCR enzyme Consider high-fidelity enzymes for accuracy, high-processivity enzymes for long templates, and hot-start enzymes for specificity
PCR	Genotype them gently Direct PCR master mix for animal-friendly genotyping
PCR	How is hot-start technology beneficial for your PCR? Primer on hot-start technology and benefits for better PCR
PCR	PCR infographic Infographic on the polymerase chain reaction
PCR	Basic principles of qRT-PCR Build a strong foundation
PCR	Perfect for fast and robust routine PCR Phire Hot Start II DNA polymerase
PCR	The power of two Fusion DNA polymerases
PCR	Optimizing T_m and annealing Using Breslauer's method
Plastics	Ultimate high-performance PCR consumables Armadillo PCR plates
Molecular cloning	Checking your T4 DNA ligase activity Two easy-to-do control experiments
Molecular cloning	The history of PCR The evolution of DNA polymerases
Molecular cloning	Molecular cloning infographic Fun facts on molecular cloning
Molecular cloning	Thermo Scientific cloning workflow Choose the right tools
Molecular cloning	5 screening methods every scientist should know Verify that your gene of interest was successfully cloned
Molecular cloning	5 cloning shortcuts from Thermo Scientific Streamline your workflow with these cloning tools
Molecular cloning	A T7 mission for high yields of RNA transcripts TranscriptAid T7 high yield transcription kit
Nucleic acid electrophoresis	Forget the powder! TopVision agarose tablets
Nucleic acid electrophoresis	8 DNA ladder tips Improve your electrophoresis results
Nucleic acid electrophoresis	Ambient shipping–decrease packaging waste Thermo Scientific DNA ladders
Restriction and modifying	176 restriction enzymes, 1 buffer FastDigest restriction enzymes
Restriction and modifying	7 common issues with restriction digestion reactions and how to avoid them