Browse and learn from our spotlight articles containing practical technical tips to help you get the meaningful results you need at the bench. This collection of articles spans PCR, molecular cloning, reverse transcription, and more. Come back often as we are always adding new content to help you advance your molecular biology applications.
Featured Spotlight articles
|Reverse transcription||Overcoming hurdles in cDNA synthesis
3 ways to boost reverse transcriptase enzyme performance
|Reverse transcription||Understanding the Molecular Evolution of a Reverse Transcriptase
Directed evolution helped build a better reverse transcriptase
|Isothermal amplification||The long and short of isothermal amplification
Learn the advantages of LAMP and WGA
|PCR||3 questions to ask when choosing a PCR enzyme
Consider high-fidelity enzymes for accuracy, high-processivity enzymes for long templates, and hot-start enzymes for specificity
|PCR||Genotype them gently
Direct PCR master mix for animal-friendly genotyping
|PCR||How is hot-start technology beneficial for your PCR?
Primer on hot-start technology and benefits for better PCR
Infographic on the polymerase chain reaction
|PCR||Basic principles of qRT-PCR
Build a strong foundation
|PCR||Perfect for fast and robust routine PCR
Phire Hot Start II DNA polymerase
|PCR||The power of two
Fusion DNA polymerases
|PCR||Optimizing Tm and annealing
Using Breslauer's method
|Plastics||Ultimate high-performance PCR consumables
Armadillo PCR plates
|Molecular cloning||Checking your T4 DNA ligase activity
Two easy-to-do control experiments
|Molecular cloning||The history of PCR
The evolution of DNA polymerases
|Molecular cloning||Molecular cloning infographic
Fun facts on molecular cloning
|Molecular cloning||Thermo Scientific cloning workflow
Choose the right tools
|Molecular cloning||5 screening methods every scientist should know
Verify that your gene of interest was successfully cloned
|Molecular cloning||5 cloning shortcuts from Thermo Scientific
Streamline your workflow with these cloning tools
|Molecular cloning||A T7 mission for high yields of RNA transcripts
TranscriptAid T7 high yield transcription kit
|Nucleic acid electrophoresis||Forget the powder!
TopVision agarose tablets
|Nucleic acid electrophoresis||8 DNA ladder tips
Improve your electrophoresis results
|Nucleic acid electrophoresis||Ambient shipping–decrease packaging waste
Thermo Scientific DNA ladders
|Restriction and modifying||176 restriction enzymes, 1 buffer
FastDigest restriction enzymes
|Restriction and modifying||7 common issues with restriction digestion reactions and how to avoid them|
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