Browse and learn from our spotlight articles containing practical technical tips to help you get the meaningful results you need at the bench. This collection of articles spans PCR, molecular cloning, reverse transcription, and more. Come back often as we are always adding new content to help you advance your molecular biology applications.
Featured Spotlight articles
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Category | Article |
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Reverse transcription | Overcoming hurdles in cDNA synthesis 3 ways to boost reverse transcriptase enzyme performance |
Reverse transcription | Understanding the Molecular Evolution of a Reverse Transcriptase Directed evolution helped build a better reverse transcriptase |
Isothermal amplification | The long and short of isothermal amplification Learn the advantages of LAMP and WGA |
PCR | 3 questions to ask when choosing a PCR enzyme Consider high-fidelity enzymes for accuracy, high-processivity enzymes for long templates, and hot-start enzymes for specificity |
PCR | Genotype them gently Direct PCR master mixes for animal-friendly genotyping |
PCR | How is hot-start technology beneficial for your PCR? Hot-start technology and benefits for better PCR |
PCR | PCR infographic Infographic on the polymerase chain reaction |
PCR | Basic principles of qRT-PCR Build a strong foundation |
PCR | Perfect for fast and robust routine PCR Phire Hot Start II DNA polymerase |
PCR | The power of two Fusion DNA polymerases |
PCR | Optimizing Tm and annealing Using the Tm calculator |
Plastics | Ultimate high-performance PCR consumables Armadillo PCR plates |
Molecular cloning | Checking your T4 DNA ligase activity Two easy-to-do control experiments |
PCR | The history of PCR The evolution of DNA polymerases |
Molecular cloning | Molecular cloning infographic Fun facts on molecular cloning |
Molecular cloning | Thermo Scientific cloning workflow Choose the right tools |
Molecular cloning | 5 screening methods every scientist should know Verify that your gene of interest was successfully cloned |
Molecular cloning | 5 cloning shortcuts from Thermo Scientific Streamline your workflow with these cloning tools |
Molecular cloning | A T7 mission for high yields of RNA transcripts TranscriptAid T7 high yield transcription kit |
Nucleic acid electrophoresis | Forget the powder! TopVision agarose tablets |
Nucleic acid electrophoresis | 8 DNA ladder tips Improve your electrophoresis results |
Nucleic acid electrophoresis | Ambient shipping–decrease packaging waste Thermo Scientific DNA ladders |
Restriction and modifying | 176 restriction enzymes, 1 buffer FastDigest restriction enzymes |
Restriction and modifying | 7 common issues with restriction digestion reactions and how to avoid them |
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