Spotlight Articles–Thermo Scientific Molecular Biology

Browse and learn from our spotlight articles containing practical technical tips to help you get the meaningful results you need at the bench. This collection of articles spans PCR, molecular cloning, reverse transcription, and more. Come back often as we are always adding new content to help you advance your molecular biology applications.

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Category Article
Reverse transcription Understanding the Molecular Evolution of a Reverse Transcriptase
Directed evolution helped build a better reverse transcriptase
Isothermal amplification The long and short of isothermal amplification
Learn the advantages of LAMP and WGA
PCR 3 questions to ask when choosing a PCR enzyme
Consider high-fidelity enzymes for accuracy, high-processivity enzymes for long templates, and hot-start enzymes for specificity
PCR Genotype them gently
Direct PCR master mix for animal-friendly genotyping
PCR How is hot-start technology beneficial for your PCR?
Primer on hot-start technology and benefits for better PCR
PCR PCR infographic
Infographic on the polymerase chain reaction
PCR Basic principles of qRT-PCR
Build a strong foundation
PCR Perfect for fast and robust routine PCR
Phire Hot Start II DNA polymerase
PCR The power of two
Fusion DNA polymerases
PCR Optimizing Tm and annealing
Using Breslauer's method
Plastics Ultimate high-performance PCR consumables
Armadillo PCR plates
Molecular cloning Checking your T4 DNA ligase activity
Two easy-to-do control experiments
Molecular cloning The history of PCR
The evolution of DNA polymerases
Molecular cloning Molecular cloning infographic
Fun facts on molecular cloning
Molecular cloning Thermo Scientific cloning workflow
Choose the right tools
Molecular cloning 5 screening methods every scientist should know
Verify that your gene of interest was successfully cloned
Molecular cloning 5 cloning shortcuts from Thermo Scientific
Streamline your workflow with these cloning tools
Molecular cloning A T7 mission for high yields of RNA transcripts
TranscriptAid T7 high yield transcription kit
Nucleic acid electrophoresis Forget the powder!
TopVision agarose tablets
Nucleic acid electrophoresis 8 DNA ladder tips
Improve your electrophoresis results
Nucleic acid electrophoresis Ambient shipping–decrease packaging waste
Thermo Scientific DNA ladders
Restriction and modifying 176 restriction enzymes, 1 buffer
FastDigest restriction enzymes
Restriction and modifying 7 common issues with restriction digestion reactions and how to avoid them

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