Online tools for analyzing primers, setting up reactions, and performing biochemical conversions & calculations.
Primer design and analysis
Analyzes the Tm, MW and extinction coefficient of a primer, and gives dilution instructions.
Analyzes different features of multiple primers simultaneously, e.g. Tm, GC content, dimer formation etc.
Reaction setup calculators
PCR reaction setup with Thermo Scientific™ Phusion™ DNA Polymerases.
qPCR reaction setup with Thermo Scientific™ DyNAmo™ qPCR Kits.
cDNA synthesis reaction setup with Thermo Scientific™ DyNAmo™ cDNA Synthesis Kit.
Calculates DNA and RNA concentrations based on measured OD260 values.
Analyzes qPCR amplification efficiency based on the slope of the standard curve.
Calculates copy numbers for DNA originating from different organisms.
Compare the error rate of Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase with other DNA polymerases.
Provides an integrated set of free tools for DNA sequence analysis, plasmid map creation, rich sequence viewing and various biochemical calculations.
For Research Use Only. Not for use in diagnostic procedures.