What percent of the product molecules contain an error after PCR (30 cycles) with different polymerases?
1 kb template
3 kb template
Phusion High-Fidelity DNA Polymerases (HF Buffer)
Phusion High-Fidelity DNA Polymerases (GC Buffer)
Pyrococcus furiosus DNA polymerase
Taq DNA polymerase
The table above demonstrates the low error rate of Phusion DNA Polymerase. After 30 cycles of PCR amplifying a 3 kb template, only 3.96 % of the product DNA molecules contain 1 (nucleotide) error each. This means that 96.04 % of the product molecules are entirely error-free. In contrast, after the same PCR protocol performed with Taq DNA polymerase, every product molecule contains an average of 2 errors.
For Research Use Only. Not for use in diagnostic procedures.